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The skeletal muscle of aged male mice exhibits a sustained growth regulatory transcriptional profile following prolonged glucocorticoid exposure compared to young males

GSE303183 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2025/11/13 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
24 samples: 6 × Tibialis Anterior, Aged vehicle, vs 6 × Tibialis Anterior, Aged Dex treatment, vs 6 × Tibialis Anterior, Young Vehicle, vs 6 × Tibialis Anterior, Young Dex treatment,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
The skeletal muscle of aged male mice exhibits sustained growth regulatory transcriptional profile following glucocorticoid exposure compared with young malesPhysiological genomics · 2025
Summary (written by the submitters)
Excess glucocorticoids induce a skeletal muscle myopathy by changing gene expression. Advanced age augments glucocorticoid-mediated muscle phenotypes, although transcriptional responses underlying those augmented phenotypes are unclear. The purpose of this study was to determine glucocorticoid-induced transcriptional landscapes in young and aged muscle following acute exposure to the hormone and following longer glucocorticoid treatment.
Read the full summary ↓
Young (4-month-old) or aged (24-month-old) male mice were administered an acute injection of dexamethasone (DEX) or vehicle or treated with DEX or vehicle for 7 days. Muscles were harvested 6.5 h after the only/last injection(s). The tibialis anterior (TA) was analyzed because mass was lower following DEX treatment only in aged males. RNA from the TA was subjected to RNA sequencing. In silico analyses determined pathways associated with glucocorticoid-sensitive genes and predicted transcription factors regulating transcriptional changes. Acute DEX altered similar numbers of genes in young (950) vs. aged males (913) although aged males had greater magnitudes of fold change. The transcriptional response in aged males following 7 days of DEX treatment was greater than acute exposure (1,196 vs 913) whereas the transcriptional response in young males was less pronounced after 7 days (599 vs 950). The glucocorticoid-sensitive genes in aged males were related to growth regulation regardless of treatment duration whereas that predominant enrichment was not evident in young males. Despite transcriptional differences, the transcription factors predicted to regulate the glucocorticoid-sensitive genes were similar in young and aged males. These data expand our understanding of transcriptional changes elicited by glucocorticoids in response to advanced age
Published in
The skeletal muscle of aged male mice exhibits sustained growth regulatory transcriptional profile following glucocorticoid exposure compared with young males
Laskin GR, Vied C, Waddell DS et al. · Physiological genomics 2025 · PMID 40880111 · doi:10.1152/physiolgenomics.00083.2025
This dataset
Open GSE303183 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE303183_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1294293 and SRA study SRP602402. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40880111) ↗
Study design
6 × Tibialis Anterior, Aged vehicle, vs 6 × Tibialis Anterior, Aged Dex treatment, vs 6 × Tibialis Anterior, Young Vehicle, vs 6 × Tibialis Anterior, Young Dex treatment,

Supports a between-group comparison across 24 samples.

How we read this: 4 replicated groups read from 24 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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