← BioTransfer GEO Dataset Finder
GEO series

Developmental manganese, but not concurrent lead exposure, alters social processing selectively in male mice

GSE303190 Mus musculus (mouse) RNA-seq (gene expression) 48 samples Submitted 2026/05/04 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 48 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
48 samples: 8 conditions, mostly with about 6 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Exposure to environmental pollutants, including heavy metals, during fetal life and early childhood, represents a major public health concern. While the health effects of exposure to a single metal have been well documented, real-life scenarios including simultaneous exposure to multiple metals have not yet been extensively investigated.
Read the full summary ↓
In this study, we investigated the neurobehavioral effects and hippocampal transcriptomic alterations in mouse offspring developmentally exposed to lead (Pb), manganese (Mn), or their combination. Female mice were exposed to environmentally relevant low doses of Pb (100 ppm), Mn (2g/L), or both, via drinking water beginning two weeks prior to conception and continuing throughout gestation and lactation. Offspring of both sexes were assessed at multiple developmental stages using a comprehensive behavioral test battery to identify age- and sex-specific effects of exposure. For transcriptomic analysis, one male and one female (two month-old) from six litters per group (naive siblings of behaviorally tested mice) were euthanized for hippocampal tissue collection. Our novel finding was a specific deficit in social recognition in adult males developmentally exposed to Mn, an effect not observed in the Pb+Mn co-exposure group. To explore potential molecular mechanisms underlying the Mn-associated behavioral phenotype in males, we focused on differentially expressed genes (DEGs) with |logFC| > 1.5 and p < 0.01. Among these, Mup3 (major urinary protein 3) emerged as particularly notable due to its role in pheromonal communication and its potential link to the altered social investigation behaviors observed in Mn-exposed males. Overall, our behavioral data suggest that developmental exposure to Pb or Mn alone, but not in combination, elicits adverse neurobehavioral outcomes, particularly in males. Importantly, the pronounced effects of individual metal exposure, along with the apparent antagonistic interaction in the combined exposure group, were mirrored at the transcriptomic level.
This dataset
Open GSE303190 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE303190_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1294306 and SRA study SRP602471. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
8 conditions, mostly with about 6 replicates each
Ippocampus, male mouse, 2 months, control ×6 Ippocampus, male mouse, 2 months, lead ×6 Ippocampus, male mouse, 2 months, manganese ×6 Ippocampus, male mouse, 2 months, lead+mangan… ×6 Ippocampus, female mouse, 2 months, control ×6 Ippocampus, female mouse, 2 months, lead ×6 Ippocampus, female mouse, 2 months, manganese ×6 Ippocampus, female mouse, 2 months, lead+mang… ×6

Supports a between-group comparison across 48 samples.

How we read this: 8 replicated groups read from 48 sample titles; they account for 48 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 8 more — browse all 48 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.