← BioTransfer GEO Dataset Finder
GEO series

Leptin as a Key Driver for Organ Fibrogenesis

GSE303498 Mus musculus (mouse) RNA-seq (gene expression) 15 samples Submitted 2025/10/03 Platform GPL34290
At a glance

RNA-seq (gene expression) · mouse · 15 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
15 samples: 5 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Leptin as a key driver for organ fibrogenesisScience advances · 2025
Summary (written by the submitters)
Leptin, a hormone primarily secreted by adipocytes, plays a central role in the regulation of energy balance and systemic metabolism through its interaction with the leptin receptor (LEPR). Beyond these functions, leptin signaling has been implicated in the pathogenesis of tissue fibrosis. Here, we report the X-ray crystal structures of a leptin-neutralizing antibody (hLep3) in the unbound and leptin-bound states.
Read the full summary ↓
The interaction of this antibody with leptin mimics the interaction of the LEPR with leptin, providing direct insights into the mechanism by which the antibody disrupts leptin signaling. We furthermore evaluate the therapeutic potential of neutralizing leptin with this antibody across distinct mouse models of fibrosis affecting the kidney, liver, lung, heart, and blood vessels, respectively. Leptin neutralization significantly inhibited fibrosis progression in all models. Mechanistically, suppression of leptin activity reduces local pro-inflammatory and pro-fibrotic processes, underscoring its therapeutic potential. These findings suggest that leptin signaling plays a crucial role in tissue fibrosis and that treatment with a leptin-neutralizing antibody may be a promising therapeutic approach.
Published in
Leptin as a key driver for organ fibrogenesis
Sun XN, Chen S, Zhao S et al. · Science advances 2025 · PMID 41124259 · doi:10.1126/sciadv.ady7904
This dataset
Open GSE303498 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE303498_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1295524 and SRA study SRP603075. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41124259) ↗
Study design
5 conditions, mostly in triplicate
Kidney_vehicle treatment ×3 Kidney_Folicaicd_IgG ×3 Kidney_Folicacid_Leptin antibody ×3 Lung_bleomycin_IgG ×3 Lung_bleomycin_Leptin antibody ×3

Supports a between-group comparison across 15 samples.

How we read this: 5 replicated groups read from 15 sample titles; they account for 15 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.