GEO series

Covalent pan-TEAD inhibitors block YAP activity and demonstrate brain penetrance in Hippo-dependent cancer models

GSE305254 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 299 samples Submitted 2026/05/07 Platform GPL24676Platform GPL16791

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 299 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Homo sapiens (human)
Samples: 299 samples: 250 distinct titles, 20 of them repeatedGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

SuperSeries — this record groups several sub-series.

Summary (written by the submitters)

This SuperSeries is composed of the SubSeries listed below.

This dataset

Open GSE305254 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE305254_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1304927 and SRA study SRP698473. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

250 distinct titles, 20 of them repeated

NGS4532 BP043209 ES-2 DMSO, ×8 NGS4532 BP043210 NCI-H226 DMSO, ×4 NGS4532 BP045307 NCI-H226 DMSO, ×4 NGS4532 BP045308 NCI-H226 DMSO, ×4 NGS4532 BP045309 ES-2 DMSO, ×4 NGS4532 BP045309 NCI-H358 DMSO, ×4 NGS4532 BP043210 NCI-H358 DMSO, ×3 NGS4532 BP045307 NCI-H358 DMSO, ×3 +12 more

Supports a case/control comparison: 27 samples read as cases, 127 as controls.

How we read this: Read from 299 sample titles: 250 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9165723 NGS4277 LIB5452716 NCI-H358 DMSO 0.02%, 48 hrs, Replicate 1
GSM9165724 NGS4277 LIB5452717 NCI-H358 DMSO 0.02%, 48 hrs, Replicate 2
GSM9165725 NGS4277 LIB5452718 NCI-H358 DMSO 0.02%, 48 hrs, Replicate 3
GSM9165726 NGS4277 LIB5452719 NCI-H358 G03498025 1uM + DMSO 0.01%, 48 hrs, Replicate 1
GSM9165727 NGS4277 LIB5452720 NCI-H358 G03498025 1uM + DMSO 0.01%, 48 hrs, Replicate 2
GSM9165728 NGS4277 LIB5452721 NCI-H358 G03498025 1uM + DMSO 0.01%, 48 hrs, Replicate 3
GSM9165729 NGS4277 LIB5452722 NCI-H358 G03427947 1uM + DMSO 0.01%, 48 hrs, Replicate 1
GSM9165730 NGS4277 LIB5452723 NCI-H358 G03427947 1uM + DMSO 0.01%, 48 hrs, Replicate 2
GSM9165731 NGS4277 LIB5452724 NCI-H358 G03427947 1uM + DMSO 0.01%, 48 hrs, Replicate 3
GSM9165732 NGS4277 LIB5452728 NCI-H358 G03498025 1uM + G03427947 1uM, 48 hrs, Replicate 1
GSM9165733 NGS4277 LIB5452729 NCI-H358 G03498025 1uM + G03427947 1uM, 48 hrs, Replicate 2
GSM9165734 NGS4277 LIB5452730 NCI-H358 G03498025 1uM + G03427947 1uM, 48 hrs, Replicate 3
GSM9165735 NGS4277 LIB5452734 NCI-H358 DMSO 0.02%, 48 hrs, Replicate 1
GSM9165736 NGS4277 LIB5452735 NCI-H358 DMSO 0.02%, 48 hrs, Replicate 2
GSM9165737 NGS4277 LIB5452736 NCI-H358 DMSO 0.02%, 48 hrs, Replicate 3
GSM9165738 NGS4277 LIB5452737 NCI-H358 G03498025 1uM + DMSO 0.01%, 48 hrs, Replicate 1
GSM9165739 NGS4277 LIB5452738 NCI-H358 G03498025 1uM + DMSO 0.01%, 48 hrs, Replicate 2
GSM9165740 NGS4277 LIB5452739 NCI-H358 G03498025 1uM + DMSO 0.01%, 48 hrs, Replicate 3
GSM9165741 NGS4277 LIB5452740 NCI-H358 G03427947 1uM + DMSO 0.01%, 48 hrs, Replicate 1
GSM9165742 NGS4277 LIB5452741 NCI-H358 G03427947 1uM + DMSO 0.01%, 48 hrs, Replicate 2
GSM9165743 NGS4277 LIB5452742 NCI-H358 G03427947 1uM + DMSO 0.01%, 48 hrs, Replicate 3
GSM9165744 NGS4277 LIB5452743 NCI-H358 G03498025 1uM + G03427947 1uM, 48 hrs, Replicate 1
GSM9165745 NGS4277 LIB5452744 NCI-H358 G03498025 1uM + G03427947 1uM, 48 hrs, Replicate 2
GSM9165746 NGS4277 LIB5452745 NCI-H358 G03498025 1uM + G03427947 1uM, 48 hrs, Replicate 3
GSM9165747 NGS4406 LIB5454997 NCI-H2122 0.02% DMSO, 48 hrs, Replicate 1
GSM9165748 NGS4406 LIB5454998 NCI-H2122 0.02% DMSO, 48 hrs, Replicate 2
GSM9165749 NGS4406 LIB5454999 NCI-H2122 0.02% DMSO, 48 hrs, Replicate 3
GSM9165750 NGS4406 LIB5455000 NCI-H2122 1 uM G03498025 + 0.01% DMSO, 48 hrs, Replicate 1
GSM9165751 NGS4406 LIB5455001 NCI-H2122 1 uM G03498025 + 0.01% DMSO, 48 hrs, Replicate 2
GSM9165752 NGS4406 LIB5455002 NCI-H2122 1 uM G03498025 + 0.01% DMSO, 48 hrs, Replicate 3
GSM9165753 NGS4406 LIB5455003 NCI-H2122 1 uM G03427947 + 0.01% DMSO, 48 hrs, Replicate 1
GSM9165754 NGS4406 LIB5455004 NCI-H2122 1 uM G03427947 + 0.01% DMSO, 48 hrs, Replicate 2
GSM9165755 NGS4406 LIB5455005 NCI-H2122 1 uM G03427947 + 0.01% DMSO, 48 hrs, Replicate 3
GSM9165756 NGS4406 LIB5455009 NCI-H2122 1 uM G03498025 + 1 uM G03427947, 48 hrs, Replicate 1
GSM9165757 NGS4406 LIB5455010 NCI-H2122 1 uM G03498025 + 1 uM G03427947, 48 hrs, Replicate 2
GSM9165758 NGS4406 LIB5455011 NCI-H2122 1 uM G03498025 + 1 uM G03427947, 48 hrs, Replicate 3
GSM9165759 NGS4406 LIB5455015 NCI-H2030 X1.1 0.02% DMSO, 48 hrs, Replicate 1
GSM9165760 NGS4406 LIB5455016 NCI-H2030 X1.1 0.02% DMSO, 48 hrs, Replicate 2
GSM9165761 NGS4406 LIB5455017 NCI-H2030 X1.1 0.02% DMSO, 48 hrs, Replicate 3
GSM9165762 NGS4406 LIB5455018 NCI-H2030 X1.1 1 uM G03498025 + 0.01% DMSO, 48 hrs, Replicate 1

+ 259 more — browse all 299 samples with per-sample file links →

Datasets on a similar topic

GSE305227 Covalent pan-TEAD inhibitors block YAP activity and demonstrate brain penetrance in Hippo-dependent cancer models [Quant-seq] 149 samples shares yap, hippo-dependent, demonstrate, inhibitors, penetrance, covalent, pan-tead, activity
GSE305224 Covalent pan-TEAD inhibitors block YAP activity and demonstrate brain penetrance in Hippo-dependent cancer models [RNA-seq] 120 samples shares yap, hippo-dependent, demonstrate, inhibitors, penetrance, covalent, pan-tead, activity
GSE330305 Covalent pan-TEAD inhibitors block YAP activity and demonstrate brain penetrance in Hippo-dependent cancer models [ATAC-seq] 30 samples shares yap, hippo-dependent, demonstrate, inhibitors, penetrance, covalent, pan-tead, activity
GSE235968 A non-canonical repressor function of JUN restrains YAP activity and suppresses YAP-dependent liver cancer growth 178 samples shares yap, activity
GSE235960 A non-canonical repressor function of JUN restrains YAP activity and suppresses YAP-dependent liver cancer growth [CUT&RUN] 64 samples shares yap, activity
GSE235965 A non-canonical repressor function of JUN restrains YAP activity and suppresses YAP-dependent liver cancer growth [iYAP5SA_10A_JUN_oe_antibodies_for_JUN_YAP_TEAD] 54 samples shares yap, activity
GSE227974 MY-COMP, an inhibitor of the transcriptional activity of YAP-B-MYB, identifies NEK2 as a novel mediator of YAP oncogenesis in uveal melanoma cells 29 samples shares yap, activity
GSE227973 MY-COMP, an inhibitor of the transcriptional activity of YAP-B-MYB, identifies NEK2 as a novel mediator of YAP oncogenesis in uveal melanoma cells [RNA-seq] 21 samples shares yap, activity

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