← BioTransfer GEO Dataset Finder
GEO series

Trem2 activation by renal tubular debris sustains Arg1+ macrophage survival and promotes tubular epithelial repair in renal ischemia–reperfusion injury

GSE306859 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/04/13 Platform GPL34290
Summary
Renal ischemia–reperfusion injury (RIRI) profoundly impairs the function of both native and transplanted kidneys. Arg1⁺ macrophages play a pivotal role in tubular regeneration and repair. The myeloid receptor triggering receptor expressed on myeloid cells 2 (Trem2) mediates the clearance of cellular debris by recognizing lipid components from injured cells. This study aimed to investigate whether Trem2 not only facilitates the debris clearance activity of Arg1⁺ macrophages in renal tubules but also sustains their survival, thereby providing a potential therapeutic target for the prevention and treatment of RIRI. To this end, we established stable RAW264.7 cell lines with Trem2 knockdown using lentivirus-mediated shRNA, followed by IL-4–induced polarization. Tubular epithelial fragments were prepared from TCMK-1 cells subjected to hypoxia/reoxygenation (H/R) and subsequently co-cultured with macrophages. RNA sequencing was performed to compare transcriptional profiles between normal Arg1⁺ macrophages and Trem2-deficient Arg1⁺ macrophages under tubular debris stimulation.
Published in
Trem2 activation by renal tubular debris sustains Arg1(+) macrophage survival and promotes tubular epithelial repair in renal ischemia-reperfusion injury
Wang J, Zhao Z, Wang R et al. · Frontiers in immunology 2026 · PMID 42039193 · doi:10.3389/fimmu.2026.1819941
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE306859_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1312665 and SRA study SRP614726. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.