Integrative bioinformatics analyses identify an essential role of ZBP1 in myocardial I/R injury

Myocardial ischemia-reperfusion (I/R) injury is the primary factor that counteracts the beneficial effects of reperfusion therapy. However, the distinct pathophysiological processes and underlying mechanisms that govern the ischemic and reperfusion phases of myocardial I/R injury remain incompletely understood. Time-series RNA-seq analyses were conducted on myocardial samples from the ischemic regions of wild-type (WT) mice after being subjected to either sham surgery or 30 minutes of ischemia followed by reperfusion (duration: 0, 1, 6, and 24 hours). Principal component analysis (PCA) demonstrated a clear segregation of gene-expression profiles between the ischemic and reperfusion stages of myocardial I/R injury. Zbp1 remained unchanged at 0 hours post-I/R injury but were consistently upregulated at 1, 6, and 24 hours post-I/R injury.