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Integrated Multi-omics Profiling of Mouse Brain Tissue Using the AllPrep Methods versus Traditional Extraction Methods

GSE308738 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/03/18 Platform GPL28330
Summary
This study systematically evaluated the AllPrep co-extraction strategy in multi-omics analysis of mouse brain tissues. At the transcriptomic level, AllPrep produced higher RNA yields and an 88.14% gene detection rate, ensuring broader coverage. In proteomics, it preserved identification depth while improving detection of membrane proteins, ubiquitin ligases, and transcription factors. Phosphoproteomics further revealed 4,347 high-confidence phosphorylation sites, including CAMK family regulatory patterns. Integrated analysis showed stronger RNA–protein correlations and enriched neuroscience-related pathways. Overall, AllPrep provides a standardized, scalable workflow that enhances data quality and supports deeper exploration of neurobiological mechanisms.
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Direct links to NCBI, no account and no request form: the whole study as GSE308738_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1332629 and SRA study SRP624782. Searching any of these in the dataset finder brings you back here.

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