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Neutrophil-induced lipoxygenase subtype promotes resolution of acute lung injury in mice

GSE309959 Mus musculus (mouse) RNA-seq (gene expression) 10 samples Submitted 2025/12/23 Platform GPL19057
At a glance

RNA-seq (gene expression) · mouse · 10 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
10 samples: 10 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Siglec-F(+) neutrophils promote the resolution of acute lung injury through ALOX15 inductionScience immunology · 2025
Summary (written by the submitters)
Neutrophils are first responders to injury, playing a crucial role in mediating early inflammation. Recently, additional roles for neutrophils have been postulated in tissue repair and resolution of inflammation. Here, we identified a distinct subset of SiglecF+ neutrophils that emerged during mouse lung injury resolution that expressed 12/15-lipoxygenase.
Read the full summary ↓
These SiglecF+ neutrophils demonstrated distinct anatomic distribution, morphology, gene expression and cell function compared to eosinophils and other neutrophil subsets. Notably, SiglecF+ neutrophils produced specialized pro-resolving mediators (SPMs) and macrophage colony-stimulating factor (M-CSF) that promoted monocyte differentiation into keratinocyte growth factor expressing-macrophages. Depletion of neutrophils hindered alveolar epithelial cell type II (AT2) repair, while adoptive transfer of SiglecF+ neutrophils accelerated epithelial restitution. Furthermore, TGF-b and GM-CSF, acting via AP-1/Jun, promoted SiglecF and 12/15-lipoxygenase expression in mouse neutrophils, and 15-lipoxygenase-1 in human neutrophils. Frequency of BAL 15-lipoxygenase+ neutrophils positively correlated with PaO2/FiO2 in ICU patients. Administration of 15-lipoxygenase-derived SPMs accelerated injury resolution and repair. Together, these findings uncover a pro-resolving subtype of neutrophils that play a pivotal role in the resolution of tissue injury.
Published in
Siglec-F(+) neutrophils promote the resolution of acute lung injury through ALOX15 induction
Tavares LP, Brüggemann TR, Cagnina RE et al. · Science immunology 2025 · PMID 41417925 · doi:10.1126/sciimmunol.aeb2657
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE309959_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1123270. Searching any of these in the dataset finder brings you back here.

Study design
10 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 10 sample titles: 10 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.