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SREBP signaling regulates lung resident memory B cell metabolism to sustain allergic memory

GSE310509 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2025/11/26 Platform GPL34475
At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
24 samples: 6 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
IgE drives allergic airway disease, yet the mechanisms sustaining IgE memory remain incompletely understood. Using a murine model of allergy, we identified CCR6⁺ CD69⁺ lung-resident memory B cells (BRM) that persist after sensitization and expand rapidly upon allergen re-exposure, generating allergen-specific IgG1 and IgE. Transcriptomic analysis revealed that BRM adopt a distinct lipid metabolic program regulated by sterol regulatory element-binding protein (SREBP) signaling.
Read the full summary ↓
Conditional deletion of this pathway revealed stage-specific roles: it is essential for GC-derived BRM formation, dispensable for maintenance, yet critical during memory recall to support BRM expansion, plasma cell differentiation, and local antibody production. Loss of SREBP signaling during memory recall impaired fatty acid metabolism, protein synthesis and effector differentiation in BRM, while also reducing plasma cell metabolic fitness, resulting in diminished IgG1 and IgE production. These findings identify a lung BRM subset as a key driver of pathogenic antibody responses and establish SREBP signaling as a stage-specific regulator of BRM, highlighting sterol pathways as potential therapeutic targets for allergic disease.
This dataset
Open GSE310509 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE310509_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1366450 and SRA study SRP646892. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
6 conditions, mostly with about 4 replicates each
WT CCR6+CD69+ csMBC ×4 WT CCR6-CD69- csMBC ×4 WT circulating csMBC ×4 SCAP cKO CCR6+CD69+ csMBC ×4 SCAP cKO CCR6-CD69- csMBC ×4 SCAP cKO circulating csMBC ×4

Supports a between-group comparison across 24 samples.

How we read this: 6 replicated groups read from 24 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.