GEO series

Alzheimer’s Disease-Associated circPDE4B Interacts with GEMIN5 to Regulate Translation

GSE311545 Homo sapiens (human) Non-coding RNA profiling 17 samples Submitted 2026/02/04 Platform GPL30173

At a glance

Non-coding RNA profiling · human · 17 samples

What it is: Non-coding RNA profilingLevels of RNAs that do not code for proteins, such as miRNA or lncRNA.
Organism: Homo sapiens (human)
Samples: 17 samples: 5 × Scrambled control IP vs 5 × circPDE4B IP vs 3 × Control Vector vs 3 × circPDE4B KD VectorGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: circPDE4B downregulation triggers GEMIN5‑dependent translational stress response and autophagy to reduce MAPT pathologyAlzheimer's & dementia : the journal of the Alzheimer's Association · 2026

Summary (written by the submitters)

In the intricate landscape of Alzheimer’s disease (AD) pathology, circular RNAs (circRNA) are emerging as influential players, orchestrating crucial aspects of gene expression, synaptic plasticity, and neuronal function. In this study, we characterize the biological role of circPDE4B, a highly abundant circRNA that is markedly downregulated in AD across brain regions.

Read the full summary ↓

Unlike most circRNAs, circPDE4B is expressed at substantially higher levels than its linear PDE4B transcript, suggesting a distinctive regulatory significance. Functional analyses in neuronal progenitor cells revealed that circPDE4B reduction triggers a translational stress response, indicating its critical role in maintaining protein synthesis homeostasis. Pulldown of circPDE4B identified the translation regulatory factor GEMIN5 as a highly specific, major interactor, and GEMIN5 knockdown showed it as a key mediator of actions of circPDE4B on translation. Loss of circPDE4B also reduced mTOR levels and subsequently enhanced autophagic flux. Notably, circPDE4B downregulation was sufficient to mitigate tau pathology in a 3D human assembloid model of tauopathy. We also identified a subset of AD-associated miRNAs interacting with circPDE4B, implicating additional layers of post-transcriptional regulation. Collectively, these findings define circPDE4B as a multifunctional regulator of neuronal homeostasis that integrates translational control, autophagy, and miRNA-mediated pathways, illuminating its potential role in the stress response and pathophysiology of AD.

Published in

circPDE4B downregulation triggers GEMIN5‑dependent translational stress response and autophagy to reduce MAPT pathology

Puri S, van der Spek SJF, Lin M et al. · Alzheimer's & dementia : the journal of the Alzheimer's Association 2026 · PMID 42080242 · doi:10.1002/alz.71436

This dataset

Open GSE311545 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE311545_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

Also filed as BioProject PRJNA1369846 and SRA study SRP649115. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42080242) ↗

Study design

5 × Scrambled control IP vs 5 × circPDE4B IP vs 3 × Control Vector vs 3 × circPDE4B KD Vector

Supports a between-group comparison across 16 samples.

How we read this: 4 replicated groups read from 17 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9326818 Scrambled control IP_rep1 Scrambled control IP
GSM9326819 Scrambled control IP_rep2 Scrambled control IP
GSM9326820 Scrambled control IP_rep3 Scrambled control IP
GSM9326821 Scrambled control IP_rep4 Scrambled control IP
GSM9326822 Scrambled control IP_rep5 Scrambled control IP
GSM9326823 circPDE4B IP_rep1 circPDE4B IP
GSM9326824 circPDE4B IP_rep2 circPDE4B IP
GSM9326825 circPDE4B IP_rep3 circPDE4B IP
GSM9326826 circPDE4B IP_rep4 circPDE4B IP
GSM9326827 circPDE4B IP_rep5 circPDE4B IP
GSM9326828 INPUT
GSM9326829 Control Vector_rep1 Control Vector
GSM9326830 Control Vector_rep2 Control Vector
GSM9326831 Control Vector_rep3 Control Vector
GSM9326832 circPDE4B KD Vector_rep1 circPDE4B KD Vector
GSM9326833 circPDE4B KD Vector_rep2 circPDE4B KD Vector
GSM9326834 circPDE4B KD Vector_rep3 circPDE4B KD Vector

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