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Single-cell RNA sequencing of Alkbh5-deficient AT2 cells Single-cell RNA sequencing of Alkbh5-deficient and control AT2 cells after bleomycin injuryafter bleomycin injury

GSE312383 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/04/29 Platform GPL24247
Summary
AT2-specific Alkbh5 knockout mice and littermate controls were administered tamoxifen to induce gene deletion. After tamoxifen induction, mice received intratracheal bleomycin to induce lung injury. Fourteen days after injury, lungs were harvested and processed into single-cell suspensions. GFP⁺ AT2 cells (reporting Sftpc-driven Cre activity) were isolated by fluorescence-activated cell sorting (FACS). To ensure optimal cell loading for the 10x Genomics Chromium platform, 40% of sorted GFP⁺ AT2 cells were mixed with 60% unsorted lung cells before encapsulation for droplet-based single-cell RNA sequencing. Libraries were prepared using the 10x Genomics Chromium Single Cell 3’ v3.1 protocol and sequenced on an Illumina platform.
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Direct links to NCBI, no account and no request form: the whole study as GSE312383_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1373211 and SRA study SRP651064. Searching any of these in the dataset finder brings you back here.

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