Distinct transcriptional landscape of IgA, IgG, and IgM ASC [RNA-Seq]

Antibody-secreting cells (ASCs) are critical effectors of humoral immunity, wherein their distinct isotypes play specialized roles in pathogen defense. Despite studies defining the transcriptional programs of selected ASC isotypes, specific mechanisms underlying isotype specific gene regulation are largely unaddressed. Here, we performed RNA-seq on surface-sorted IgM, IgG, and IgA ASCs isolated from the spleens and mediastinal lymph nodes of mice following influenza infection. We observed isotype-specific expression patterns that indicate distinct functions underpin ASC of each isotype.