Distinct chromatin accessibility landscape of IgA, IgG, and IgM ASC [ASC.ATACseq]

Antibody-secreting cells (ASCs) are critical effectors of humoral immunity, wherein their distinct isotypes play specialized roles in pathogen defense. Despite studies defining the transcriptional programs of selected ASC isotypes, specific mechanisms underlying isotype specific gene regulation are largely unaddressed. Here, we performed ATAC-seq on surface-sorted IgM, IgG, and IgA ASCs isolated from the spleens and mediastinal lymph nodes of mice following influenza infection. We observed isotype-specific chromatin accessibility patterns that indicate distinct functions underpin ASC of each isotype.