RNA-Seq analysis of Mapk14 knockout and myogenic differentiation in C2C12 cells
RNA-seq (gene expression) · mouse · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 12 samples: 3 × WT C2C12 cells, DM. biol vs 3 × WT C2C12 cells, GM. biol vs 3 × MAPK14 knockout C2C12 cells, DM. biol vs 3 × MAPK14 knockout C2C12 cells, GM. biolGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- p38 MAPK orchestrates cross-tissue potassium homeostasis for survivalNature communications · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE315765_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1378441 and SRA study SRP653900. Searching any of these in the dataset finder brings you back here.
Supports a case/control comparison: 6 samples read as cases, 6 as controls.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM9436742 WT C2C12 cells, DM. biol rep 1 WT C2C12 cells, DM. biol
- GSM9436743 WT C2C12 cells, DM. biol rep 2 WT C2C12 cells, DM. biol
- GSM9436744 WT C2C12 cells, DM. biol rep 3 WT C2C12 cells, DM. biol
- GSM9436745 WT C2C12 cells, GM. biol rep 1 WT C2C12 cells, GM. biol
- GSM9436746 WT C2C12 cells, GM. biol rep 2 WT C2C12 cells, GM. biol
- GSM9436747 WT C2C12 cells, GM. biol rep 3 WT C2C12 cells, GM. biol
- GSM9436748 MAPK14 knockout C2C12 cells, DM. biol rep 1 MAPK14 knockout C2C12 c…
- GSM9436749 MAPK14 knockout C2C12 cells, DM. biol rep 2 MAPK14 knockout C2C12 c…
- GSM9436750 MAPK14 knockout C2C12 cells, DM. biol rep 3 MAPK14 knockout C2C12 c…
- GSM9436751 MAPK14 knockout C2C12 cells, GM. biol rep 1 MAPK14 knockout C2C12 c…
- GSM9436752 MAPK14 knockout C2C12 cells, GM. biol rep 2 MAPK14 knockout C2C12 c…
- GSM9436753 MAPK14 knockout C2C12 cells, GM. biol rep 3 MAPK14 knockout C2C12 c…
- GSE312993 Transcriptome analysis (bulk RNAseq) of FlncKO-C2C12 during myogenic differentiation 39 samples shares c2c12, myogenic
- GSE316686 Total RNA-seq of C2C12 myotubes treated with agrin or MuSK antibodies 20 samples shares c2c12, rna-seq
- GSE309939 Modulating MyoD1 dosage activates alternate cell fate beyond myogenic differentiation [RNA-seq] 47 samples shares myogenic, rna-seq
- GSE263080 RNA-sea of Smad4 wild-type or Smad4 knockout KPC PDAC organoids from monocultures or co-cultures with pancreatic stellate cells and RNA-seq of flow-sorted PSCs from the same co-cultures. 44 samples shares knockout, rna-seq
- GSE325832 RNA-seq analysis of skeletal muscle from control (Cre) and Terf2 knockout (KO) mice in gastrocnemius-plantaris-soleus (GPS) and tibialis anterior (TA) 35 samples shares knockout, rna-seq
- GSE217662 Decoding Trp53 regulatory program with knockout cell transcriptomic landscape analyses [scRNA-seq] 247 samples shares knockout, rna-seq
- GSE292739 RNA-seq analysis in hepatocyte-specific Atg7-knockout mice with different p62 mutations 21 samples shares knockout, rna-seq
- GSE272532 Bulk RNA-seq analysis of WT and tristetraprolin (TTP)-knockout basophils treated with antigen/IgE stimulation 18 samples shares knockout, rna-seq
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.