GEO series

Sensory innervation induced by osteoblast-derived netrin-4 attenuates bone loss in aged mice

GSE317225 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2026/01/27 Platform GPL13112

Summary

The sensory denervation has a causal role in bone loss. The mechanism of bone cells regulation of sensory nerve wiring and sprouting for maintaining bone homeostasis remains unclear. Here we found that netrin-4, secreted by osteoblasts, facilitates the sprouting of sensory nerves through the DCC receptor and plays a regulatory role in bone formation. Decreased bone formation is coupled with reduced sensory innervation in aged mice and ablation of sensory nerves recapitulates this phenotype. Both mice and human data reveal that netrin-4 is positively correlated with bone volume, which expression was not affected by ablation of sensory nerves in mice. The sensory sprouting and bone formation are inhibited by siDCC, a netrin-4 receptor antagonist, in vivo and in vitro. Specifically, knockout of the netrin4 gene in the osteoblastic cells significantly reduces both sensory innervation and bone volume in adult mice. Thus, we show that sensory innervation induced by osteoblast-derived netrin-4 preserve bone homeostasis and attenuates bone loss in aged mice.

This dataset

Open GSE317225 in finder → Find more mouse RNA-seq datasets →

Download

Direct links to NCBI, no account and no request form: the whole study as GSE317225_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1406306 and SRA study SRP665365. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 9 more — browse all 9 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.