Bulk RNA-seq of young HSCs and Selp high vs Selp low HSCs in pre-aged mice

Hematopoietic stem cells (HSCs) undergo functional and molecular alterations during aging that impair regenerative capacity and bias lineage output. P-selectin (Selp/CD62P) expression has been associated with inflammatory and stress-responsive hematopoietic states, but its transcriptional impact within the HSC compartment during early aging remains incompletely defined. In this study, we performed bulk RNA sequencing of phenotypically defined HSCs isolated from young mice and from pre-aged mice in which HSCs were further stratified based on Selp surface expression (Selp_high and Selp_low). Comparative transcriptomic analysis was conducted to identify gene expression programs associated with early aging and Selp-associated heterogeneity within the HSC pool. These data provide a resource for understanding molecular pathways linked to HSC functional decline and inflammatory priming during the initial stages of hematopoietic aging.