Analysis of gene expression in primary human foreskin keratinocytes (HFK) +/- HPV16 or HPV6 E7
RNA-seq (gene expression) · human · 15 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 15 samples: 15 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Inactivation of the RB1 and PTPN14 tumor suppressors cooperatively enables the carcinogenic activity of the human papillomavirus E7 oncoproteinbioRxiv : the preprint server for biology · 2026
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE319393_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1423225 and SRA study SRP676820. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 15 sample titles: 15 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM9517247 GFP_A
- GSM9517248 GFP_B
- GSM9517249 GFP_C
- GSM9517250 HPV16E7_wt_A
- GSM9517251 HPV16E7_wt_B
- GSM9517252 HPV16E7_wt_C
- GSM9517253 HPV16_DLYC_A
- GSM9517254 HPV16_DLYC_B
- GSM9517255 HPV16_DLYC_C
- GSM9517256 HPV6E7_wt_A
- GSM9517257 HPV6E7_wt_B
- GSM9517258 HPV6E7_wt_C
- GSM9517259 HPV6_GLHC_A
- GSM9517260 HPV6_GLHC_B
- GSM9517261 HPV6_GLHC_C
- GSE324594 Transcriptomic changes in primary human keratinocytes following exposure to lamotrigine, lamotrigine-N2-oxide, lamotrigine in combination with valproic acid or interferon-γ, and interferon-γ alone 36 samples shares keratinocytes, primary
- GSE315599 Primary human fibroblasts and keratinocytes stimulated with IL-17A and/or TNF 32 samples shares keratinocytes, primary
- GSE280620 Bulk RNA-seq of primary keratinocytes treated with IgG from patients with bullous pemphigoid or controls II 14 samples shares keratinocytes, primary
- GSE280619 Bulk RNA-seq of primary keratinocytes treated with IgG from patients with bullous pemphigoid or controls I 8 samples shares keratinocytes, primary
- GSE213737 Stimulation with THBS4 activates pathways that regulate proliferation, migration and inflammation in primary human keratinocytes 6 samples shares keratinocytes, primary
- GSE28552 Expression profiles of human primary keratinocytes differentiated with TPA 7 samples shares keratinocytes, primary
- GSE293618 Higher expression of HPV16 derived E7_LI Transcript Observed in Men with HIV and Recurrent Anal Cancer 12 samples shares hpv16
- GSE294286 Effect of IRF3 knockdown on gene expression in an HPV16-containing epithelium 12 samples shares hpv16
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.