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Bulk RNA sequencing study of iGCB day3 cells to investigate differences between Eif3e cg1cre knockout B cells and control B cells

GSE320249 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/05/01 Platform GPL24247
Summary
To obtain a larger number of Eif3e-deficient B cells induced by cg1cre, we utilized an in vitro germinal center B cell (iGCB) culture system. By day 3 of culture, we harvested abundant YFP+ Eif32-deficient B cells and their corresponding control cells. RNA-seq comparison revealed that Eif3e-deficient B cells upregulated some co-stimulatory molecules, including Cd80 and Icam1. GO enrichment analysis showed that upregulated genes were primarily associated with ribosome-related pathways, while downregulated genes were mainly enriched in lipid metabolism. The upregulation of co-stimulatory molecules may confer Eif3e-deficient B cells with the capacity to promote CD4+ T cell activation.
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Also filed as BioProject PRJNA1424683. Searching any of these in the dataset finder brings you back here.

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