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Hyperoxia-induced lung microvascular injury is mediated by endothelial cell-derived WNT5A

GSE320519 Mus musculus (mouse) RNA-seq (gene expression) 9 samples Submitted 2026/05/27 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 9 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
9 samples: 3 × RA vs 3 × HO vs 3 × HO-WNT5AGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Hyperoxia-induced lung microvascular injury is mediated by endothelial cell-derived WNT5AJournal of thoracic disease · 2026
Summary (written by the submitters)
Background: Bronchopulmonary dysplasia (BPD) is a common complication in preterm infants, with dysmorphic microvascular development being a key factor in the pathogenesis of BPD. WNT5A plays a role in angiogenesis and vascular development and is closely associated with lung disease. However, the role and mechanism of WNT5A in hyperoxia-induced pulmonary microvascular impairment remain unclear.
Read the full summary ↓
Methods: A hyperoxia exposure mouse model (85% oxygen for 14 days) simulating preterm BPD was used, and was sampled on postnatal day (P) 3, 7, and 14. Western blot (WB) was used to detect the expression of endothelial cell markers von Willebrand factor (vWF) and WNT5A. Mouse pulmonary microvascular endothelial cells (PMVECs) and human umbilical vein endothelial cells (HUVECs) were cultured in 85% oxygen for 48 hours. WNT5A expression and secretion were measured through WB and ELISA. WNT5A inhibitors or recombinant WNT5A (rWNT5A) were co-cultured with cells, and the cell function was assessed. Total RNA was extracted from PMVECs, followed by RNA sequencing. Results: WNT5A expression was decreased in the lungs of hyperoxia-exposed mice at P7. Consistently, in vitro hyperoxia exposure reduced WNT5A expression and its downstream effector CaMKIIγ in endothelial cells compared with normoxia-exposed controls. Both WNT5A inhibition and rWNT5A changed the migration and tube formation functions of HUVECs in normoxic and hyperoxic conditions. RNA sequencing revealed differentially expressed genes (DEGs), and suggested enrichment in the ErbB, MAPK, and Hippo pathways in the hyperoxia group. Conclusions: Hyperoxia decreased the WNT5A expression and impaired endothelial cell function potentially through WNT5A-CaMKIIγ.
Published in
Hyperoxia-induced lung microvascular injury is mediated by endothelial cell-derived WNT5A
Ye W, Bu X, Zhou J · Journal of thoracic disease 2026 · PMID 42182624 · doi:10.21037/jtd-2025-1-2699
This dataset
Open GSE320519 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE320519_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1428763 and SRA study SRP679523. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42182624) ↗
Study design
3 × RA vs 3 × HO vs 3 × HO-WNT5A

Supports a between-group comparison across 9 samples.

How we read this: 3 replicated groups read from 9 sample titles; they account for 9 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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