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Postnatal Pax7-expressing limb cells are multipotent and generate non-myogenic lineages that persist into adulthood.

GSE324349 Mus musculus (mouse) RNA-seq (gene expression) 25 samples Submitted 2026/07/27 Platform GPL30172Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 25 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
25 samples: 7 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Organs are composed of diverse cell types that arise from distinct or shared progenitor cells. Skeletal muscle originates from mesoderm-derived Pax7⁺ stem/progenitor cells, which typically differentiate into myoblasts and form muscle fibers. During embryonic development, however, Pax7⁺ somitic cells can also generate non-muscle lineages such as dermis and adipocytes.In this study, we examined whether Pax7⁺ cells in postnatal growing limb muscle retain similar multipotency.
Read the full summary ↓
Lineage-tracing analyses revealed unexpected plasticity during the early postnatal period, with Pax7⁺ cells giving rise not only to myogenic cells but also to multiple non-myogenic lineages. Among these, we identified a previously unrecognized Pax7-derived fibro-adipogenic progenitor population (Pax7FAPs). Using mouse models, we further found that Notch signaling promotes a fibrogenic molecular program in neonatal Pax7⁺ cells, at the expense of myogenic differentiation, potentially biasing their fate toward the Pax7FAP lineage. Long-term tracing showed that Pax7-derived FAPs generated during the neonatal stage persist into adulthood and display enhanced proliferative capacity following muscle injury. Moreover, adult muscle injury can also induce the generation of Pax7FAPs, which proliferate more robustly than resident stromal cells. Together, these findings reveal previously unappreciated multipotency in postnatal Pax7⁺ cells and provide new insights into their contribution to muscle development and regeneration.
This dataset
Open GSE324349 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE324349_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1434451 and SRA study SRP682329. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
7 conditions, mostly with about 4 replicates each
Postnatal Day3, whole muscle Tibialis Anterio ×4 Postnatal Day3, Pax7 Progeny cells ×4 Postnatal Day10, Pax7 Progeny cells ×4 Postnatal Day22-1, Pax7 Progeny cells ×4 Postnatal Day7, Pax7-tdTomato-Cells ×2 Postnatal Day7, Pax7-NICD-Cells ×2 Long-chase Pax7 lineage (2mo) ×2 Postnatal Day22-2, Pax7 Progeny cells ×1 +2 more

Supports a between-group comparison across 22 samples.

How we read this: 7 replicated groups read from 25 sample titles; they account for 22 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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