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miR-151-5p Regulates Neural Stem Cell Fate by Targeting Aph1a to Modulate Notch Signaling Gradients

GSE327779 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/04/16 Platform GPL9185
Summary
To investigate the role of miR-151-5p in regulating neural stem cell (NSC) fate during neocortical development, we performed RNA-seq profiling of NSCs isolated from the developing mouse cerebral cortex. Conditional knockout of miR-151-5p led to increased Sox2 expression and enhanced NSC proliferation. Mechanistically, Aph1a, a core subunit of the γ-secretase complex, was identified as a direct target of miR-151-5p. Overexpression of Aph1a phenocopied the knockout phenotype, promoting NSC proliferation via elevated NICD levels. Our results demonstrate that miR-151-5p biases NSC fate specification by targeting Aph1a to modulate Notch signaling, thereby fine-tuning the balance between NSC maintenance and differentiation. This study reveals a novel miR-151-5p/Aph1a/Notch signaling axis governing NSC fate, providing a critical post-transcriptional regulatory layer in mammalian neocortical development.
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Also filed as BioProject PRJNA1452805 and SRA study SRP691309. Searching any of these in the dataset finder brings you back here.

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