GEO series

Limonin ameliorates metabolic dysfunction-associated steatohepatitis in mice

GSE328199 Mus musculus (mouse) RNA-seq (gene expression) 11 samples Submitted 2026/04/30 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 11 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 11 samples: 6 × 8-week MCD diet combined with 4-week intragastric administration of limonin, vs 5 × 8-week MCD diet,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Limonin (Lim), a triterpenoid predominantly enriched in citrus fruits, exhibits diverse pharmacological activities, yet its therapeutic efficacy and mechanistic basis in metabolic dysfunction-associated steatohepatitis (MASH) remain poorly defined. Here, we show that Lim attenuates liver fibrosis and hepatocellular apoptosis in MASH mouse models induced by a methionine–choline-deficient (MCD) diet.

This dataset

Open GSE328199 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE328199_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1454326 and SRA study SRP692344. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

6 × 8-week MCD diet combined with 4-week intragastric administration of limonin, vs 5 × 8-week MCD diet,

Supports a between-group comparison across 11 samples.

How we read this: 2 replicated groups read from 11 sample titles; they account for 11 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9674824 8-week MCD diet, rep1 8-week MCD diet,
GSM9674825 8-week MCD diet, rep2 8-week MCD diet,
GSM9674826 8-week MCD diet, rep3 8-week MCD diet,
GSM9674827 8-week MCD diet, rep4 8-week MCD diet,
GSM9674828 8-week MCD diet, rep5 8-week MCD diet,
GSM9674829 8-week MCD diet combined with 4-week intragastric administration of limonin, rep1 8-week MCD diet combine…
GSM9674830 8-week MCD diet combined with 4-week intragastric administration of limonin, rep2 8-week MCD diet combine…
GSM9674831 8-week MCD diet combined with 4-week intragastric administration of limonin, rep3 8-week MCD diet combine…
GSM9674832 8-week MCD diet combined with 4-week intragastric administration of limonin, rep4 8-week MCD diet combine…
GSM9674833 8-week MCD diet combined with 4-week intragastric administration of limonin, rep5 8-week MCD diet combine…
GSM9674834 8-week MCD diet combined with 4-week intragastric administration of limonin, rep6 8-week MCD diet combine…

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.