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Effect of interferon-gamma stimulation on the transcriptome of RANKL/M-CSF-induced osteoclasts

GSE329318 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/05/02 Platform GPL34328
Summary
Interferon-gamma (IFN-γ) is a pleiotropic cytokine with context-dependent effects on bone homeostasis, capable of both inhibiting and promoting osteoclastogenesis depending on the cellular milieu and stage of differentiation. To delineate the direct transcriptional consequences of IFN-γ exposure during terminal osteoclast differentiation, we performed RNA sequencing on RANKL/M-CSF-induced osteoclasts treated with or without IFN-γ. Differential expression analysis identified a suite of IFN-γ-responsive genes and revealed significant perturbation of pathways governing osteoclast fusion and bone resorption. Notably, we identified Lipocalin-2 (Lcn2) as a key downstream effector whose expression is robustly induced by IFN-γ. These data elucidate the molecular mechanisms by which IFN-γ translates immune signals into pro-osteoclastogenic instructions, highlighting a specific transcriptional program that enhances osteoclast differentiation and may contribute to inflammatory bone loss.
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Direct links to NCBI, no account and no request form: the whole study as GSE329318_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1458653 and SRA study SRP695557. Searching any of these in the dataset finder brings you back here.

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