GEO series

Transcriptomics Analysis of bulk liver RNA from 129/Sv-Cyp2e1tm1Gonz/J (CYP2E1-KO) and 129S1/SvImJ (WT) mice

GSE329473 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/06/01 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 12 samples: 3 × Female WT Liver, vs 3 × Female KO Liver, vs 3 × Male WT Liver, vs 3 × Male KO Liver,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

To establish the consequences of whole-animal deletion of CYP2E1, we isolated RNA from bulk liver tissue of 16-week male and female WT and KO mice. Mice were maintained on a normal chow diet and were euthanized using CO2 and cervical dislocation prior to liver dissection.

This dataset

Open GSE329473 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE329473_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1459310 and SRA study SRP695953. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

3 × Female WT Liver, vs 3 × Female KO Liver, vs 3 × Male WT Liver, vs 3 × Male KO Liver,

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9704838 Female WT Liver, Rep 1 Female WT Liver,
GSM9704839 Female WT Liver, Rep 2 Female WT Liver,
GSM9704840 Female KO Liver, Rep 1 Female KO Liver,
GSM9704841 Female KO Liver, Rep 2 Female KO Liver,
GSM9704842 Female KO Liver, Rep 3 Female KO Liver,
GSM9704843 Male WT Liver, Rep 1 Male WT Liver,
GSM9704844 Male KO Liver, Rep 1 Male KO Liver,
GSM9704845 Male WT Liver, Rep 2 Male WT Liver,
GSM9704846 Male WT Liver, Rep 3 Male WT Liver,
GSM9704847 Female WT Liver, Rep 3 Female WT Liver,
GSM9704848 Male KO Liver, Rep 2 Male KO Liver,
GSM9704849 Male KO Liver, Rep 3 Male KO Liver,

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Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.