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Bulk RNA-seq of juvenile mouse liver following NDMA or saline treatment in WT and Mgmt-/- genotypes across multiple timepoints

GSE332646 Mus musculus (mouse) RNA-seq (gene expression) 214 samples Submitted 2026/05/22 Platform GPL19057
At a glance

RNA-seq (gene expression) · mouse · 214 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
214 samples: 40 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Transcriptomic profiling of mouse liver tissue to characterize the acute and long-term gene expression response to the carcinogen N-nitrosodimethylamine (NDMA) in juvenile mice. RaDR+/+;GPT+/+ (WT) and RaDR+/+;GPT+/+;Mgmt-/- (Mgmt knockout) mice received intraperitoneal injections of NDMA (3.5 mg/kg at postnatal day 8 and 7 mg/kg at postnatal day 15) or saline vehicle. Liver tissue was collected at Day 1, Day 2, Day 5, Week 10, and Month 10 post-treatment and subjected to bulk RNA-seq to identify differential gene expression patterns across genotypes, sexes, treatments, and timepoints.
This dataset
Open GSE332646 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE332646_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1468715 and SRA study SRP702445. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
40 conditions, mostly with about 4 replicates each
WT_M_P8P15NDMA_Day1_D ×13 WT_F_P8P15NDMA_Day1_D ×13 WT_F_P8P15Saline_Day1_D ×12 WT_M_P8P15Saline_Day1_D ×9 WT_M_P8P15NDMA_Month10_D ×7 Mgmt_F_P8P15Saline_Month10_D ×7 Mgmt_F_P8P15NDMA_Month10_D ×7 Mgmt_M_P8P15Saline_Day1_D ×6 +32 more

Supports a between-group comparison across 214 samples.

How we read this: 40 replicated groups read from 214 sample titles; they account for 214 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 174 more — browse all 214 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.