Bulk RNA-seq of juvenile mouse liver following NDMA or saline treatment in WT and Mgmt-/- genotypes across multiple timepoints

Transcriptomic profiling of mouse liver tissue to characterize the acute and long-term gene expression response to the carcinogen N-nitrosodimethylamine (NDMA) in juvenile mice. RaDR+/+;GPT+/+ (WT) and RaDR+/+;GPT+/+;Mgmt-/- (Mgmt knockout) mice received intraperitoneal injections of NDMA (3.5 mg/kg at postnatal day 8 and 7 mg/kg at postnatal day 15) or saline vehicle. Liver tissue was collected at Day 1, Day 2, Day 5, Week 10, and Month 10 post-treatment and subjected to bulk RNA-seq to identify differential gene expression patterns across genotypes, sexes, treatments, and timepoints.