Mouse embryo transcriptomes after two-cell blastomere separation

This study profiled mouse preimplantation embryos by Smart-seq2 RNA-seq to assess whether separation of late two-cell blastomeres alters the developmental transcriptomic program. The dataset includes control 8-cell embryos, split morula-stage embryos derived from two-cell blastomeres, control morula embryos, split blastocyst-stage embryos derived from two-cell blastomeres, and control blastocysts. The data were used to compare global transcriptomic similarity, developmental-stage effects, sample correlations, hierarchical clustering and pseudotime trajectories between split embryos and intact controls.