GEO series

Ferritin-mediated iron homeostasis regulates fibroblast activation and shapes post-infarction cardiac remodeling

GSE330351 Mus musculus (mouse) Single-cell RNA-seq 9 samples Submitted 2026/06/30 Platform GPL28330

At a glance

Single-cell RNA-seq · mouse · 9 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism: Mus musculus (mouse)
Samples: 9 samples: 9 × RNA-seq of heartGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Ferritin-mediated iron homeostasis regulates fibroblast activation and shapes post-infarction cardiac remodelingThe Journal of nutritional biochemistry · 2026

Summary (written by the submitters)

Myocardial infarction (MI) triggers dynamic tissue remodeling in which fibroblast activation critically determines scar formation and long-term cardiac function. Although iron dysregulation has been implicated in acute myocardial injury, its role in non-cardiomyocyte remodeling remains incompletely understood. Here, we integrated cross-species transcriptomics, spatial transcriptomics, single-cell RNA sequencing, and functional studies to investigate iron metabolic remodeling after MI.

Read the full summary ↓

We found that ferritin heavy chain (Fth1) was robustly induced in the infarct region during the proliferative phase after MI and was predominantly localized to macrophages and fibroblasts. Single-cell trajectory analysis revealed that high Fth1 expression characterized early or inflammatory fibroblast states, whereas progressive Fth1 downregulation accompanied differentiation into extracellular matrix–producing myofibroblasts. Functionally, ferritin depletion promoted fibroblast activation in both neonatal rat and adult mouse cardiac fibroblasts, accompanied by expansion of the intracellular labile iron pool and enhanced profibrotic gene expression. Mechanistically, intracellular iron chelation suppressed fibroblast activation and attenuated FoxO1 signaling–associated transcriptional programs. Notably, temporally targeted treatment with the cell-permeable iron chelator 2,2′-bipyridyl (BPD) during the proliferative phase (days 3–14), but not the acute inflammatory phase (days 0-3), effectively attenuated pathological fibrosis and improved cardiac function after MI. Transcriptomic profiling further showed that iron chelation modulated both fibroblast activation–associated and inflammatory gene programs within the infarcted myocardium. Collectively, these findings identify ferritin-regulated iron homeostasis as a key determinant of fibroblast plasticity and highlight phase-specific intracellular iron modulation as a potential therapeutic strategy for limiting adverse cardiac remodeling after MI.

Published in

Ferritin-mediated iron homeostasis regulates fibroblast activation and shapes post-infarction cardiac remodeling

Jiang M, Liu T, Shi J et al. · The Journal of nutritional biochemistry 2026 · PMID 42323100 · doi:10.1016/j.jnutbio.2026.110451

This dataset

Open GSE330351 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE330351_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1463690 and SRA study SRP698656. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42323100) ↗

Study design

9 × RNA-seq of heart

Supports a between-group comparison across 9 samples.

How we read this: 1 replicated groups read from 9 sample titles; they account for 9 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9724298 RNA-seq of heart_1 RNA-seq of heart
GSM9724299 RNA-seq of heart_2 RNA-seq of heart
GSM9724300 RNA-seq of heart_3 RNA-seq of heart
GSM9724301 RNA-seq of heart_4 RNA-seq of heart
GSM9724302 RNA-seq of heart_5 RNA-seq of heart
GSM9724303 RNA-seq of heart_6 RNA-seq of heart
GSM9724304 RNA-seq of heart_7 RNA-seq of heart
GSM9724305 RNA-seq of heart_8 RNA-seq of heart
GSM9724306 RNA-seq of heart_9 RNA-seq of heart

Datasets on a similar topic

GSE319401 Exercise Mitigates Cardiac Dysfunction Post-MI in Ovariectomized Mice via miR-21/Sox7-Mediated Suppression of Cardiac Fibroblast Activation 24 samples shares fibroblast, activation, cardiac
GSE267256 Bulk RNA-seq analysis reveals the crucial role of Collagen triplex helix repeat containing-1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction 17 samples shares fibroblast, remodeling, cardiac
GSE306718 Macrophage Cluster of Differentiation 163 promotes post-infarction cardiac repair and preserves left ventricular function via osteopontin 37 samples shares post-infarction, cardiac
GSE336247 Cardiac fibroblast deletion of PTBP1 modulates the profibrotic response by alternative splicing II 36 samples shares fibroblast, cardiac
GSE269634 Triggering AHR resolves TGF-β1 induced fibroblast activation and promotes AT1 cell regeneration in alveolar organoids 32 samples shares fibroblast, activation
GSE279927 RNA next-generation sequencing of cardiac fibroblasts isolated from young and old mice 7 days post-MI 25 samples shares fibroblast, cardiac
GSE266899 The GLP-1R agonist semaglutide reshapes pancreatic cancer associated fibroblasts reducing collagen hydroxyprolination and favoring immune infiltration. 18 samples shares fibroblast, shapes
GSE305170 Maintenance of Fibroblast Identity During Cardiac Reprogramming and Heart Repair [RNA-seq] 18 samples shares fibroblast, cardiac

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.