← BioTransfer GEO Dataset Finder
GEO series

Hippocampal APOE/S1P axis regulates synaptic and behavioral deficits in a mouse model of autism

GSE332712 Mus musculus (mouse) RNA-seq (gene expression) 9 samples Submitted 2026/05/26 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 9 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
9 samples: 3 × MAN@SKI II treated (BTBR) mouse hippocampus, 6 weeks, vs 3 × Con (C57BL/6J) mouse hippocampus, 6 weeks, vs 3 × Model (BTBR) mouse hippocampus, 6 weeks,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Autism spectrum disorder (ASD) is a neurodevelopmental condition of unknown mechanism. Synaptic pathology is a core feature, but the key molecular regulators are unclear. The brain's major apolipoprotein APOE transports lipids including S1P to regulate neuronal function; however, the role of the APOE/S1P axis in ASD is unknown. Here, we found that serum APOE and S1P were elevated in children with ASD.
Read the full summary ↓
In the BTBR mouse hippocampus, both were also elevated, with abnormal APOE aggregation on neurons. To test causality, we developed a brain-targeted nanomicelle (MAN@SKI II) that inhibits hippocampal S1P. MAN@SKI II lowered APOE/S1P levels, reactivated the PI3K/Akt/mTOR pathway, repaired synaptic ultrastructure, and improved social and cognitive behaviors in BTBR mice. Together, these findings indicate that the APOE/S1P axis plays a regulatory role in hippocampal synaptic pathology and behavioral deficits in ASD, suggesting a potential mechanistic target for therapeutic intervention.
This dataset
Open GSE332712 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE332712_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1468970 and SRA study SRP702578. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
3 × MAN@SKI II treated (BTBR) mouse hippocampus, 6 weeks, vs 3 × Con (C57BL/6J) mouse hippocampus, 6 weeks, vs 3 × Model (BTBR) mouse hippocampus, 6 weeks,

Supports a between-group comparison across 9 samples.

How we read this: 3 replicated groups read from 9 sample titles; they account for 9 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.