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RNA‑seq transcriptomic analysis of HL‑1 cardiomyocytes under OGD/R injury with or without Phillyrin treatment

GSE333299 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/05/31 Platform GPL24247
Summary
Ischemia‑reperfusion (I/R) injury is a key pathological process in myocardial infarction, for which effective therapies are still lacking. Phillyrin has been reported to exert cardioprotective effects, but its molecular mechanisms remain unclear. HL‑1 mouse cardiomyocytes were subjected to oxygen‑glucose deprivation/reoxygenation (OGD/R) to mimic I/R injury. Cells were divided into two groups: OGD/R alone (IR) and OGD/R with Phillyrin treatment (IR+Phil). Total RNA was extracted from 3 biological replicates per group and sequenced using Illumina NovaSeq 6000. Transcriptomic profiling identified differentially expressed genes and pathways regulated by OGD/R and Phillyrin treatment. The processed data file contains raw read counts and FPKM values for all genes across all samples. This dataset provides a comprehensive transcriptomic resource to explore the molecular mechanisms of Phillyrin‑mediated cardioprotection against I/R injury.
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Direct links to NCBI, no account and no request form: the whole study as GSE333299_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1470647 and SRA study SRP703777. Searching any of these in the dataset finder brings you back here.

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