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Bulk RNA-seq of EO771 breast cancer cells with 3 conditions

GSE335950 Mus musculus Expression profiling by high throughput sequencing 9 samples 2026/06/24 GPL30172
Summary
Bulk RNA sequencing was performed to compare mRNA expression profiles across six experimental conditions: monoculture of EO771 breast cancer cells; conditioned medium from DRG neurons applied to EO771 cells; and direct co-culture of EO771 and DRG neurons followed by fluorescence-activated cell sorting (FACS) to isolate EO771 cell populations. The aim was to identify transcriptional programs regulated by tumor–neuron interactions, including acute gene expression changes, intercellular signaling pathways, and broader pathway-level alterations. The dataset is intended for differential expression analysis, pathway enrichment, and hypothesis generation for downstream functional studies.
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