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Lipofected mRNAs are spliced in Human cells

GSE341263 Homo sapiens (human) RNA-seq (gene expression) 38 samples Submitted 2026/07/24 Platform GPL34295
At a glance

RNA-seq (gene expression) · human · 38 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
38 samples: 18 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Current strategies for therapeutic use of mRNAs and for testing the function of specific mRNA isoforms assumes that transfected mRNAs are not further processed by cellular splicing machinery. Here we show that lipofected mRNAs can be spliced in human cells, utilizing canonical splice donor and acceptor sites and this is suppressed by spliceosome inhibition. This post-transcriptional splicing has important strategic implications for the use of transfected mRNAs in experiments and therapies.
This dataset
Open GSE341263 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE341263_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1500039 and SRA study SRP720971. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
18 conditions, mostly in duplicate
HEK293T transfected 250ng CTDSP2 bicistronic … ×3 HEK293T transfected 250ng HOXB7 bicistronic r… ×3 HEK293T transfected 250ng BCAP29 bicistronic … ×2 HEK293T transfected 250ng CANX bicistronic re… ×2 HEK293T transfected 250ng PHIP bicistronic re… ×2 HEK293T transfected 250ng HSP90AA1 bicistroni… ×2 HEK293T transfected 250ng CANX bicistronic re… ×2 HEK293T transfected 250ng CANX bicistronic re… ×2 +10 more

Supports a between-group comparison across 38 samples.

How we read this: 18 replicated groups read from 38 sample titles; they account for 38 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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