Transcriptomics analysis of gene expression in normal and METTL3 or WTAP deficient Human HeLa cells
RNA-seq (gene expression) · human · 6 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 6 samples
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE50582_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA217982 and SRA study SRP029515. Searching any of these in the dataset finder brings you back here.
The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.
+ 6 more — browse all 6 samples with per-sample file links →
- GSE300197 The cytoskeleton drives abnormal chromatin-lamina interactions in LMNA-deficient cardiomyocytes. 30 samples shares deficient, normal
- GSE234322 The effect of METTL3 RNA methyltransferase inhibition on breast cancer cell gene expression 18 samples shares mettl3
- GSE268352 ZC3H13 and WTAP KO in hMDMs multi stimuli(NGS5421) 48 samples shares wtap
- GSE312942 Inhibition of METTL3 by STC-15 induces RNA misprocessing that results in dsRNA formation and activates innate immunity [RNA-seq] 36 samples shares mettl3
- GSE274217 METTL3-dependent m6A RNA methylation represses transposable elements derived enhancers to suppress naïve pluripotency in human pluripotent stem cells [RNA-Seq] 28 samples shares mettl3
- GSE313114 Inhibition of METTL3 by STC-15 induces RNA misprocessing that results in dsRNA formation and activates innate immunity [RNA-Seq dose_response] 21 samples shares mettl3
- GSE298180 METTL3-dependent m6A methylation in atherogenic endothelial inflammation [RNA] 9 samples shares mettl3
- GSE285328 Spatial control of m6A deposition on enhancer and promoter RNAs through co-acetylation of METTL3 and H3K27 on chromatin [RNA-seq] 6 samples shares mettl3
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.