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Epigenomic Signatures of Neuronal Diversity in the Mammalian Brain

GSE63137 Mus musculus Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Methylation profiling by high throughput sequencing 31 samples Submitted 2015/06/17 Platform GPL13112Platform GPL17021
Summary
We developed an affinity purification approach to isolate tagged nuclei in mice (similar to INTACT; [Deal R.B. and Henikoff S. A simple method for gene expression and chromatin profiling of individual cell types within a tissue. Dev. Cell 18,1030-1040. (2010)]) and used it to characterize genome-wide patterns of transcription, DNA methylation, and chromatin accessibility in 3 major neuron classes of the neocortex (excitatory pyramidal neurons, parvalbumin (PV)-positive GABAergic interneurons, and vasoactive intestinal peptide (VIP)-positive GABAergic interneurons). By combining cell purification and integrative analysis, our findings relate the phenotypic and functional complexity of neocortical neurons to their underlying transcriptional and epigenetic diversity.
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Direct links to NCBI, no account and no request form: the whole study as GSE63137_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 31 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA266828 and SRA study SRP049674. Searching any of these in the dataset finder brings you back here.

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