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Cells release subpopulations of exosomes with distinct molecular and functional properties

GSE72351 Mus musculus (mouse) Expression microarray 9 samples Submitted 2016/04/30 Platform GPL17400
At a glance

Expression microarray · mouse · 9 samples

What it is
Expression microarrayGene activity read on a chip (the older technology that RNA-seq replaced).
Organism
Mus musculus (mouse)
Samples
9 samples
Data you can get
A processed expression table (series matrix) and the array files, from NCBI.
Summary (written by the submitters)
Cells release nano-sized membrane vesicles that are involved in intercellular communication by transferring biological information between cells. It is generally accepted that cells release at least three types of these extracellular vesicles (EVs): apoptotic bodies, microvesicles and exosomes. Whilst exosomes are assumed to be a homogenous population of EVs, they have a wide range of putative functions.
Read the full summary ↓
Therefore, we hypothesized that cells release subpopulations of exosomes with distinct molecular compositions and functional properties. Exosomes were isolated from conditioned medium by differential ultracentrifugation. Sucrose density gradient centrifugation of the resulting exosome pellet revealed the presence of two distinct subpopulations, one smaller, slow migrating population (HD-Exo), and one fast migrating, larger population (LD-Exo). Interestingly, the exosome subpopulations were found to have differential effects on gene expression in recipient endothelial cells. In conclusion, we demonstrate that cells release distinct subpopulations of exosomes with unique biophysical properties and molecular compositions that elicit differential effects on recipient cells. Our data supports the heterogeneous nature of exosomes. It also highlights the importance of better discrimination between subpopulations to allow more detailed studies on exosome biology and function, and assist in the development of exosome-based diagnostics and therapeutics.
This dataset
Download · which file do I need?

Direct links to NCBI, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need.

All supplementary files ↗
3 · The original raw files

Whole study · RAW.tar

Everything the authors deposited as one archive (GSE72351_RAW.tar), including the raw array or instrument files. Can be large; the picker in step 2 takes just the samples you need.

What the files usually look like: For arrays the series matrix is normally the normalised expression table you want; the raw array files (CEL and similar) are in the RAW.tar.

Also filed as BioProject PRJNA293861. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 9 more — browse all 9 samples with per-sample file links →

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