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Single-cell indexed RNA-Seq of human hematopoietic stem and progenitors

GSE75478 Homo sapiens Expression profiling by high throughput sequencing 2414 samples Submitted 2017/01/19 Platform GPL11154
Summary
To characterize early human hematopoiesis on a single-cell level we developed an approach termed index-omics, which combines flow-cytometric, single-cell transcriptomic and single-cell lineage fate data. Healthy human bone marrow was labeled with a panel of up to 11 FACS surface markers commonly used to identify human hematopoietic stem and progenitor cells (HSPCs). Lin-CD34+38+ progenitors and Lin-CD34+CD38- stem cell enriched HSPCs were individually sorted, their surface marker fluorescence intensities recorded, and subjected to single-cell RNAseq or single-cell ex vivo cultures.
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Direct links to NCBI, no account and no request form: the whole study as GSE75478_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 2414 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA304408 and SRA study SRP066781. Searching any of these in the dataset finder brings you back here.

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