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Microglia development follows a stepwise program to regulate brain homeostasis - Single Cell RNA-seq

GSE79818 Mus musculus Expression profiling by high throughput sequencing 18 samples Submitted 2016/07/13 Platform GPL19057
Summary
Microglia play important roles in life-long brain maintenance and in pathology, but are also crucial in the developing central nervous system; yet their regulatory dynamics during development have not been fully elucidated. Genome-wide chromatin and expression profiling coupled with single-cell transcriptomic analysis throughout development reveal that microglia undergo three temporal developmental stages in synchrony with the brain: early, pre-, and adult microglia, which are under the control of distinct regulatory circuits. Knockout of the transcription factor MafB caused disruption of homeostasis in adulthood and increased inflammation. Environmental perturbations, such as the microbiome or prenatal immune activation, led to dysregulation of the developmental program, particularly in terms of inflammation. Together, our work identifies a stepwise developmental program of microglia integrating immune response pathways that may be associated with several neurodevelopmental disorders.
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Direct links to NCBI, no account and no request form: the whole study as GSE79818_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 18 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA317060 and SRA study SRP072734. Searching any of these in the dataset finder brings you back here.

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