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Total RNA expression in mouse heart tissues after injections of VEGFA-containing extracellular vessicle, lipidnanoparticles and naked-VEGFA mRNA

GSE220060 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2026/06/18 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
18 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Targeted Delivery of mRNA to the Heart via Extracellular Vesicles or Lipid NanoparticlesJournal of extracellular vesicles · 2026
Summary (written by the submitters)
The study used the next-generation sequencing to compare mRNA content of mouse heart tissue after direct injection of different VEGFA-containining extracellular vesicles (EVs), lipidnanoparticles (LNPs) or naked VEGFA-mRNA to untreated control. The EVs used were originated from human lung epithelial (HTB), umbilical vein endothelial (HUVEC), or cardiac progenitor cells (CPCs) in which the cells were treated with LNPs containing VEGFA prior to the collection of EVs, fractions of the VEGFA-mRNA ended up in the EVs.
Read the full summary ↓
Our goal is to carry out functional and pathway analysis of the differentially expressed genes. The RNA profiles of EV content from heart tissues were generated by RNA-Seq using Illumina NovaSeq6000. Differentially expressed genes were identified using the DESeq2 package. We found that the VEGFA mRNA was among the most upregulated genes in the injected hearts. We also identified multiple genes and pathways that are significantly associated with angiogenesis and cardiac conditions. Inflamatory response genes and pathways were upregulated by the LNPs and naked-mRNA injection to a greater extend compared to EV injection. The result suggested that the injection of EVs caused less transcriptional changes in the heart tissue.
Published in
Targeted Delivery of mRNA to the Heart via Extracellular Vesicles or Lipid Nanoparticles
Nawaz M, Tangruksa B, Heydarkhan-Hagvall S et al. · Journal of extracellular vesicles 2026 · PMID 42275464 · doi:10.1002/jev2.70324
This dataset
Open GSE220060 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE220060_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA908734 and SRA study SRP411308. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42275464) ↗
Study design
6 conditions, mostly in triplicate
Mouse heart tissue, untreated, ×3 Mouse heart tissue, treated with HTB-Evs, ×3 Mouse heart tissue, treated with HUVEC-Evs, ×3 Mouse heart tissue, treated with CPC-Evs, ×3 Mouse heart tissue, treated with LNPs, ×3 Mouse heart tissue, treated with naked-VEGFA, ×3

Supports a between-group comparison across 18 samples.

How we read this: 6 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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