Synthetic IL-21 cytokine mimetic promotes T cell function
Single-cell RNA-seq · mouse · 12 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Mus musculus (mouse)
- Samples
- 12 samples: 12 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Design of a potent interleukin-21 mimic for cancer immunotherapyScience immunology · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE240834_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1005330 and SRA study SRP455025. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 12 sample titles: 12 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM7710260 PBS rep 1 GEX
- GSM7710261 Neo2 GEX
- GSM7710262 21h10 rep 1 GEX
- GSM7710263 PBS rep 1 HTO
- GSM7710264 Neo2 HTO
- GSM7710265 21h10 rep 1 HTO
- GSM7710266 PBS rep 2 GEX
- GSM7710267 21h10 rep 2 GEX
- GSM7710268 IL21 GEX
- GSM7710269 PBS rep 2 HTO
- GSM7710270 21h10 rep 2 HTO
- GSM7710271 IL21 HTO
- GSE327851 Transneuronal cytokine delivery promotes functional recovery across spinal cord contusion severities via descending circuit plasticity 9 samples shares cytokine, promotes
- GSE278050 KLF13 promotes SLE Pathogenesis by modifying chromatin accessibility of key proinflammatory cytokine genes [RNA-seq] 42 samples shares cytokine, promotes
- GSE280837 IL-21 Shapes the B Cell Response in a Context-Dependent Manner [CUT&RUN] 13 samples shares il-21
- GSE228457 IL-7 mediated immunoregulatory feedback loop promotes dampening of immune effector functions by hypoinflammatory monocytes. 305 samples shares promotes
- GSE230838 Loss of cisPTase function in the liver promotes a highly penetrant form of fatty liver disease that rapidly transitions to hepatocellular carcinoma [RNA-seq] 17 samples shares promotes
- GSE252337 Dysfunction of infiltrating cytotoxic CD8+ T cells within the graft promotes murine kidney allotransplant tolerance 16 samples shares promotes
- GSE289751 Resolution of inflammation in imprinted fibroblast-like synoviocytes establishes a transcriptomic signature that promotes endothelial cell dysfunction. 12 samples shares promotes
- GSE303699 Autoimmune Disease Risk Gene ANKRD55 Promotes Th17 Effector Function Through Metabolic Modulation [RNA-Seq] 60 samples shares promotes
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.