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Sequencing to determine the effect of neuronal IL-1 receptor knockout and microglia depletion on stress-induced changes to the neuronal transcriptome

GSE253687 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/03/01 Platform GPL24247
Summary
Chronic stress is associated with increased anxiety, cognitive deficits, and post-traumatic stress disorder. Repeated social defeat (RSD) in mice causes long-term stress-sensitization associated with increased microglia activation, monocyte accumulation, and enhanced interleukin (IL)-1 signaling in endothelia and neurons. With stress-sensitization, mice have amplified neuronal, immune, and behavioral responses to acute stress 24d later. This is clinically relevant as it shares key aspects with post-traumatic-stress-disorder. The mechanisms underlying stress-sensitization are unclear, but enhanced fear memory may be critical. The purpose of this study was to determine the influence of microglia and IL-1R1 signaling in neurons in the development of sensitization and increased fear memory after RSD. The goal of this study was to sequence nuclei from the hippocampus to determine downstream pathways of neuronal IL-1R1 (nIL-1R1) and microglia reactivity.
Published in
Novel microglial transcriptional signatures promote social and cognitive deficits following repeated social defeat
Goodman EJ, DiSabato DJ, Sheridan JF et al. · Communications biology 2024 · PMID 39341879 · doi:10.1038/s42003-024-06898-9
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Also filed as BioProject PRJNA1066764 and SRA study SRP484759. Searching any of these in the dataset finder brings you back here.

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