Transcriptome profile of C2C12 wild-type and Fam134b knockout myoblasts and differentiated myotubes
RNA-seq (gene expression) · mouse · 16 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 16 samples: 4 × WT_UNDIFF vs 4 × WT_DIFF vs 4 × KO_UNDIFF vs 4 × KO_DIFFGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Two FAM134B isoforms differentially regulate ER dynamics during myogenesisThe EMBO journal · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE254885_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1072140 and SRA study SRP487557. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 16 samples.
How we read this: 4 replicated groups read from 16 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8059792 WT_UNDIFF_1 WT_UNDIFF
- GSM8059793 WT_UNDIFF_2 WT_UNDIFF
- GSM8059794 WT_UNDIFF_3 WT_UNDIFF
- GSM8059795 WT_UNDIFF_4 WT_UNDIFF
- GSM8059796 WT_DIFF_1 WT_DIFF
- GSM8059797 WT_DIFF_2 WT_DIFF
- GSM8059798 WT_DIFF_3 WT_DIFF
- GSM8059799 WT_DIFF_4 WT_DIFF
- GSM8059800 KO_UNDIFF_1 KO_UNDIFF
- GSM8059801 KO_UNDIFF_2 KO_UNDIFF
- GSM8059802 KO_UNDIFF_3 KO_UNDIFF
- GSM8059803 KO_UNDIFF_4 KO_UNDIFF
- GSM8059804 KO_DIFF_1 KO_DIFF
- GSM8059805 KO_DIFF_2 KO_DIFF
- GSM8059806 KO_DIFF_3 KO_DIFF
- GSM8059807 KO_DIFF_4 KO_DIFF
- GSE194370 RNAseq of 3 and 5 days differentiated C2C12 cells treated with conditioned medium of KPC, 4662, LLC or C26 cells 42 samples shares c2c12, differentiated
- GSE333910 Transcriptomic profiling of C2C12 myotubes treated with D-galactose and red ginseng-derived interventions 30 samples shares c2c12, myotubes
- GSE300503 Differentiation and Dexamethasone Atrophy on C2C12 myotubes 24 samples shares c2c12, myotubes
- GSE316686 Total RNA-seq of C2C12 myotubes treated with agrin or MuSK antibodies 20 samples shares c2c12, myotubes
- GSE302488 PFOS perturbs metabolism in C2C12 skeletal myoblasts 12 samples shares c2c12, myoblasts
- GSE315765 RNA-Seq analysis of Mapk14 knockout and myogenic differentiation in C2C12 cells 12 samples shares c2c12, knockout
- GSE314495 effect of depletion of Znf143 on gene expression of C2C12 myoblasts with or without induction for myogenic differentiation 12 samples shares c2c12, myoblasts
- GSE294869 The effect of carnitine treatment on palmitic acid-treated C2C12 myotubes 9 samples shares c2c12, myotubes
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.