Bulk RNA-seq of immunopanned astrocytes with the treatment of Shh and Fgf

Purpose: Examine differential gene expression changes in in vitro astrocytes with the administration of Shh agonist and Fgf protein vs control; Methods: Astrocytes were purified using a previously published immunopanning protocol, SAG-Shh agonist and DMSO are added into cell culture medium for 3 days, and Fgf2 and Fgf10 were added to medium respectively for 7 days, PBS treated astrocytes as controls. RNA-seq were perfomed (Lexongen QuantSeq3'mRNA-SEQ V2 Library prep kit) on all different conditions; Results: We observed differential gene expression changes in SAG treated conditions compared to WT, but did not observe a significant number of genes changes in Fgf treated groups compared to controls.