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RNA-seq analysis of hypothalamus in female mice reveals effects of membrane-initiated estrogen receptor alpha signaling

GSE328990 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/04/27 Platform GPL24247
Summary
Membrane-initiated estrogen receptor alpha (mERα) signaling plays an important role in bone metabolism, but its contribution in the brain remains unclear. In this study, we performed RNA sequencing of hypothalamic tissue from 16-week-old female mice with selective inactivation of mERα signaling in POMC neurons (POMC-C451Af/f) and wild-type littermate controls. Total RNA was isolated from hypothalamus, and samples were pooled (two animals per sample) resulting in four biological replicates per genotype. Libraries were prepared using poly(A) selection and sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads. Transcript abundance was quantified using Salmon and summarized to gene-level counts. Differential expression analysis was performed using DESeq2. This dataset provides insight into transcriptional changes associated with disrupted mERα signaling in hypothalamic neurons.
Published in
Membrane-initiated estrogen receptor-α signaling in the hypothalamus regulates trabecular bone in femur in female mice
Jiang Y, Horkeby K, Henning P et al. · The Journal of endocrinology 2026 · PMID 42133461 · doi:10.1530/JOE-25-0462
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Also filed as BioProject PRJNA1457336 and SRA study SRP694470. Searching any of these in the dataset finder brings you back here.

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