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Glycolytic activity instructs germ layer proportions through regulation of Nodal and Wnt signaling

GSE282050 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/03/14 Platform GPL24247
Summary
Metabolic pathways can influence cell fate decisions, but their regulative role during embryonic development remains poorly understood. Here, we demonstrate a crucial role of glycolytic activity in regulating signalling pathways involved in mesoderm and endoderm specification. Using an mESC-based in vitro model for gastrulation, we found that glycolysis inhibition increases ectodermal cell fates at the expense of mesodermal and endodermal lineages. We used single cell RNA sequencing (scRNA-seq) to analyze the mechanistic insight into the nutritional regulation of cell fate decision making.
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Glycolytic activity instructs germ layer proportions through regulation of Nodal and Wnt signaling
Stapornwongkul KS, Hahn E, Poliński P et al. · Cell stem cell 2025 · PMID 40245870 · doi:10.1016/j.stem.2025.03.011
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Also filed as BioProject PRJNA1186714 and SRA study SRP545510. Searching any of these in the dataset finder brings you back here.

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