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Bulk RNA-seq analysis of molecular characteristics induced by Cr1l over-expression in B16mhgp100 subcutaneous implanted tumors

GSE291584 Mus musculus Expression profiling by high throughput sequencing 7 samples Submitted 2025/07/04 Platform GPL24247
Summary
Secreted proteins are central mediators of intercellular communications and can serve as therapeutic targets in diverse diseases. The ~1903 human genes encoding secreted proteins are difficult to study through common genetic approaches. To address this hurdle and, more generally, to discover cancer therapeutics, we developed the Cancer Immunology Data Engine (CIDE, https://cide.ccr.cancer.gov), which incorporates 90 omics datasets spanning 8575 tumor profiles with immunotherapy outcomes from 17 solid tumor types. CIDE systematically identifies all genes associated with immunotherapy outcomes. Then, we focused on secreted proteins prioritized by CIDE without known cancer roles and validated regulatory effects on immune checkpoint blockade for AOAH, CR1L, COLQ, and ADAMTS7 in mouse models. The top hit, AOAH (Acyloxyacyl Hydrolase), potentiates immunotherapies in multiple tumor models by sensitizing T-cell receptors to weak antigens and protecting dendritic cells through depleting immunosuppressive arachidonoyl phosphatidylcholines and oxidized derivatives.
Published in
Cancer immunology data engine reveals secreted AOAH as a potential immunotherapy
Gong L, Luo J, Yang E et al. · Cell 2025 · PMID 40730154 · doi:10.1016/j.cell.2025.07.004
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Direct links to NCBI, no account and no request form: the whole study as GSE291584_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1234189 and SRA study SRP569310. Searching any of these in the dataset finder brings you back here.

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