GEO series

Astrocytic FKBP5 regulates neuroinflammation and cognitive outcomes in male mouse models of excitotoxic epilepsy

GSE293536 Mus musculus (mouse) RNA-seq (gene expression) 20 samples Submitted 2026/02/02 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 20 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 20 samples: 5 × WT, saline_7d vs 5 × WT, LPS_7d vs 5 × Fkbp5-KO, saline_7d vs 5 × Fkbp5-KO, LPS_7dGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Astrocytic FKBP5 regulates neuroinflammation and cognitive outcomes in male mouse models of excitotoxic epilepsyBrain, behavior, and immunity · 2026

Summary (written by the submitters)

FK506-binding protein 51 (FKBP51, encoded by FKBP5) is a multisignaling cochaperone that regulates cellular stress responses and inflammatory signaling through the NF-κB pathway. Although FKBP51 is upregulated in reactive astrocytes, its role in epilepsy and excitotoxic neuroinflammation remains unknown. Excessive astrogliosis and impaired glutamate transporter-1 (GLT-1)-mediated glutamate clearance promote excitotoxicity and increase seizure susceptibility.

Read the full summary ↓

Here, we investigated how both global and astrocyte-specific Fkbp5 deletions influence seizure susceptibility, astrogliosis, neuroinflammation, and cognition in male mice subjected to a kainic acid (KA)-induced epilepsy mouse model. Global Fkbp5 knockout (Fkbp5-KO) presented lower seizure activity along with decreased neuronal loss and astrogliosis in the hippocampus compared with the wild-type mice. Astrocyte-specific Fkbp5 conditional knockout (aFkbp5-cKO) mice similarly attenuated seizure severity, decreased astrogliosis, improved novel object recognition, and preserved GLT-1 expression in hippocampal CA3. Glia-neuron mixed cultures derived from Fkbp5-KO brains showed reduced NMDA-induced neurotoxicity and astrogliosis, accompanied by decreased NF-κB p65 phosphorylation. Notably, overexpression of an Fkbp5 quadruple mutant that disrupts the FKBP51-NF-κB interaction inhibited proinflammatory lipopolysaccharide-induced astrogliosis and NF-κB activation. Transcriptomic analysis of Fkbp5-KO hippocampi further confirmed suppression of NF-κB-driven inflammatory pathways. In summary, astrocytic FKBP51 mediates reactive astrogliosis and GLT-1 downregulation, linking excitotoxic neuroinflammation with seizure susceptibility and cognitive impairment, and represents a potential intervention target for epilepsy.

Published in

Astrocytic FKBP5 regulates neuroinflammation and cognitive outcomes in male mouse models of excitotoxic epilepsy

Gan YL, Lin SH, Kang YP et al. · Brain, behavior, and immunity 2026 · PMID 41534726 · doi:10.1016/j.bbi.2026.106278

This dataset

Open GSE293536 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE293536_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1244893 and SRA study SRP575732. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41534726) ↗

Study design

5 × WT, saline_7d vs 5 × WT, LPS_7d vs 5 × Fkbp5-KO, saline_7d vs 5 × Fkbp5-KO, LPS_7d

Supports a between-group comparison across 20 samples.

How we read this: 4 replicated groups read from 20 sample titles; they account for 20 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8884790 WT, saline_7d_1 WT, saline_7d
GSM8884791 WT, saline_7d_2 WT, saline_7d
GSM8884792 WT, saline_7d_3 WT, saline_7d
GSM8884793 WT, saline_7d_4 WT, saline_7d
GSM8884794 WT, saline_7d_5 WT, saline_7d
GSM8884795 WT, LPS_7d_1 WT, LPS_7d
GSM8884796 WT, LPS_7d_2 WT, LPS_7d
GSM8884797 WT, LPS_7d_3 WT, LPS_7d
GSM8884798 WT, LPS_7d_4 WT, LPS_7d
GSM8884799 WT, LPS_7d_5 WT, LPS_7d
GSM8884800 Fkbp5-KO, saline_7d_1 Fkbp5-KO, saline_7d
GSM8884801 Fkbp5-KO, saline_7d_2 Fkbp5-KO, saline_7d
GSM8884802 Fkbp5-KO, saline_7d_3 Fkbp5-KO, saline_7d
GSM8884803 Fkbp5-KO, saline_7d_4 Fkbp5-KO, saline_7d
GSM8884804 Fkbp5-KO, saline_7d_5 Fkbp5-KO, saline_7d
GSM8884805 Fkbp5-KO, LPS_7d_1 Fkbp5-KO, LPS_7d
GSM8884806 Fkbp5-KO, LPS_7d_2 Fkbp5-KO, LPS_7d
GSM8884807 Fkbp5-KO, LPS_7d_3 Fkbp5-KO, LPS_7d
GSM8884808 Fkbp5-KO, LPS_7d_4 Fkbp5-KO, LPS_7d
GSM8884809 Fkbp5-KO, LPS_7d_5 Fkbp5-KO, LPS_7d

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.