Slow RNAPII elongation facilitates early pluripotency cell transitions without reducing replication fork rates
RNA-seq (gene expression) · mouse · 17 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 17 samples: 8 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Slow RNAPII elongation enhances naive pluripotency rewiring while maintaining high replication fork speedScience advances · 2026
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE293593_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1245343 and SRA study SRP575992. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 17 samples.
How we read this: 8 replicated groups read from 17 sample titles; they account for 17 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8886420 WT mESCs, RNAseq 0 days 2i, replicate 1 WT mESCs, RNAseq 0 days…
- GSM8886421 WT mESCs, RNAseq 0 days 2i, replicate 2 WT mESCs, RNAseq 0 days…
- GSM8886422 WT mESCs, RNAseq 1 days 2i, replicate 1 WT mESCs, RNAseq 1 days…
- GSM8886423 WT mESCs, RNAseq 1 days 2i, replicate 2 WT mESCs, RNAseq 1 days…
- GSM8886424 WT mESCs, RNAseq 2 days 2i, replicate 1 WT mESCs, RNAseq 2 days…
- GSM8886425 WT mESCs, RNAseq 2 days 2i, replicate 2 WT mESCs, RNAseq 2 days…
- GSM8886426 WT mESCs, RNAseq 7 days 2i, replicate 1 WT mESCs, RNAseq 7 days…
- GSM8886427 WT mESCs, RNAseq 7 days 2i, replicate 2 WT mESCs, RNAseq 7 days…
- GSM8886428 RNAPII-mut mESCs, RNAseq 0 days 2i, replicate 1 RNAPII-mut mESCs, RNAse…
- GSM8886429 RNAPII-mut mESCs, RNAseq 0 days 2i, replicate 2 RNAPII-mut mESCs, RNAse…
- GSM8886430 RNAPII-mut mESCs, RNAseq 1 days 2i, replicate 1 RNAPII-mut mESCs, RNAse…
- GSM8886431 RNAPII-mut mESCs, RNAseq 1 days 2i, replicate 2 RNAPII-mut mESCs, RNAse…
- GSM8886432 RNAPII-mut mESCs, RNAseq 1 days 2i, replicate 3 RNAPII-mut mESCs, RNAse…
- GSM8886433 RNAPII-mut mESCs, RNAseq 2 days 2i, replicate 1 RNAPII-mut mESCs, RNAse…
- GSM8886434 RNAPII-mut mESCs, RNAseq 2 days 2i, replicate 2 RNAPII-mut mESCs, RNAse…
- GSM8886435 RNAPII-mut mESCs, RNAseq 7 days 2i, replicate 1 RNAPII-mut mESCs, RNAse…
- GSM8886436 RNAPII-mut mESCs, RNAseq 7 days 2i, replicate 2 RNAPII-mut mESCs, RNAse…
- GSE233975 DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID 45 samples shares rnapii, elongation
- GSE290944 DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID. [ChIP-seq] 24 samples shares rnapii, elongation
- GSE259426 miR-203 facilitates timely cell fate transitions via epigenetic modulation during early embryogenesis (RNA-Seq) 27 samples shares facilitates, transitions
- GSE294457 TCA cycle rewiring underpins histone acetylation sourcing and cell-fate transitions during exit from naive pluripotency 12 samples shares pluripotency, transitions
- GSE259428 miR-203 facilitates timely cell fate transitions via epigenetic modulation during early embryogenesis 39 samples shares facilitates, transitions
- GSE61995 m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation (m6a-seq) 18 samples shares pluripotency, facilitates
- GSE200103 CK2 phosphorylation of cap methyltransferase CMTR1 promotes RNAPII interaction 12 samples shares rnapii
- GSE274544 Single cell profiling reveals three endothelial to hematopoietic transitions with divergent isoform expression landscapes [scRNA-Seq] 1494 samples shares transitions
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.