GEO series

The outcomes of caloric restriction are driven by enhanced glucocorticoid rhythms.

GSE298149 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 16 samples Submitted 2025/11/04 Platform GPL24247

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 16 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Mus musculus (mouse)
Samples: 16 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Hepatic metabolic reprogramming in male mice during short-term caloric restriction involves enhanced glucocorticoid rhythmsNature communications · 2025

Summary (written by the submitters)

Caloric restriction extends lifespan and healthspan across species, with feeding times synchronized to circadian rhythms further maximizing its benefits. However, the mechanisms linking diet, diurnal rhythms, and lifespan are not fully understood. In mice, the time point most strongly tied to dietary effects on lifespan coincides with the peak of glucocorticoid secretion (ZT12, lights-off).

Read the full summary ↓

Caloric restriction raises circulating glucocorticoid hormone levels, but their functional relevance remains untested. We show that the glucocorticoid receptor (GR) is critical for the effects of caloric restriction. Hepatocyte-specific GR mutant mice fail to respond to caloric restriction indicating that increased glucocorticoid amplitudes support its benefits. Using multiomics techniques in murine liver, we find that nutrient deprivation elicits a nuclear switch from active STAT signaling to increased FOXO1 activity, enabling the GR to activate a unique diet-specific gene expression program. Taken together, our results suggest that glucocorticoid rhythms are crucial for caloric restriction-induced metabolic reprogramming.

Published in

Hepatic metabolic reprogramming in male mice during short-term caloric restriction involves enhanced glucocorticoid rhythms

Makris K, Fonda V, Ramadhani FF et al. · Nature communications 2025 · PMID 41381594 · doi:10.1038/s41467-025-67228-z

This dataset

Open GSE298149 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE298149_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1268427 and SRA study SRP587854. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41381594) ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 16 more — browse all 16 samples with per-sample file links →

Datasets on a similar topic

GSE248866 The outcomes of caloric restriction are driven by enhanced glucocorticoid rhythms. 114 samples shares glucocorticoid, restriction, outcomes, enhanced, caloric, rhythms, driven
GSE248864 Hepatic Glucocorticoid Receptor (GR) drives the diurnal adaptation to Caloric Restriction 90 samples shares glucocorticoid, restriction, caloric
GSE284988 Epigenetic profiling of hematopoietic stem cells identifies KDR and PU.1 as regulators of aging transcriptome and caloric restriction response 58 samples shares restriction, caloric
GSE296874 RNA-Seq analysis of high-fiber diets and caloric restriction in mouse liver 36 samples shares restriction, caloric
GSE330380 The effects of adiponectin knockout on hepatic gene expression in male and female C57BL/6NCrl mice fed ad libitum or undergoing caloric restriction 22 samples shares restriction, caloric
GSE250376 Epigenomic and Functional Convergence Between Glucocorticoid- and IL4-Driven Macrophage Programming 13 samples shares glucocorticoid, driven
GSE306265 Astrocyte glucocorticoid receptor signaling restricts neuronal plasticity 106 samples shares glucocorticoid
GSE232089 The glucocorticoid receptor potentiates aldosterone-induced transcription by the mineralocorticoid receptor 77 samples shares glucocorticoid

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.