← BioTransfer GEO Dataset Finder
GEO series

Effect of JAM3 knockdown on glioma cell transcriptome

GSE302919 Homo sapiens (human) RNA-seq (gene expression) 20 samples Submitted 2025/07/24 Platform GPL16791
At a glance

RNA-seq (gene expression) · human · 20 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
20 samples: 5 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Junctional adhesion molecule C limits glioblastoma stem-like cell invasion by regulating integrin adhesion at the endothelial interfaceCell reports · 2025
Summary (written by the submitters)
The JamC adhesion molecule plays a potential role in glioblastoma stem cell adhesion and interaction with the vascular niche. To study this, we performed JamC knock-out (KO) in GSC1 and GSC4 cells and analyzed transcriptomic changes using 3’ end RNA-seq (3’SRP).
Published in
Junctional adhesion molecule C limits glioblastoma stem-like cell invasion by regulating integrin adhesion at the endothelial interface
Rosińska S, André-Grégoire G, Kerhervé M et al. · Cell reports 2025 · PMID 40875295 · doi:10.1016/j.celrep.2025.116194
This dataset
Open GSE302919 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE302919_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1292838 and SRA study SRP601572. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40875295) ↗
Study design
5 conditions, mostly with about 4 replicates each
GSC1_NC1 ×4 GSC1_KO110 ×4 GSC4_NC1 ×4 GSC4_KO409 ×4 GSC4_KO404 ×4

Supports a between-group comparison across 20 samples.

How we read this: 5 replicated groups read from 20 sample titles; they account for 20 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.