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RNA-seq of gubernaculum and testis from wild-type, cetacean INSL3 knock-in, and INSL3 knockout mice across developmental stages

GSE305702 Mus musculus (mouse) RNA-seq (gene expression) 39 samples Submitted 2025/10/28 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 39 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
39 samples: 13 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Evolutionary relaxation and functional change of INSL3 and RXFP2 may underlie natural cryptorchidism in mammalsEMBO reports · 2025
Summary (written by the submitters)
This dataset contains high-throughput RNA sequencing (RNA-seq) data generated from gubernaculum and testis samples of wild-type (WT), cetacean INSL3 knock-in (KI), and INSL3 knockout (KO) mice at selected postnatal developmental stages. The purpose of the study was to investigate the molecular consequences of replacing the endogenous mouse Insl3 coding sequence with the cetacean INSL3 ortholog, and to compare transcriptomic profiles among WT, KI, and KO mice to elucidate the functional role of INSL3 in testicular descent and cryptorchidism.
Read the full summary ↓
RNA-seq was performed on gubernaculum tissue at postnatal day 8 (P8) and testis tissue at P8, P23, and P40 (with some groups excluded due to degeneration or non-specific damage). The dataset supports integrative analyses of developmental stage–specific and genotype–specific gene expression changes in reproductive tissues.
Published in
Evolutionary relaxation and functional change of INSL3 and RXFP2 may underlie natural cryptorchidism in mammals
Zheng Y, Chai S, Zhong C et al. · EMBO reports 2025 · PMID 41219539 · doi:10.1038/s44319-025-00636-w
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE305702_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1307492 and SRA study SRP610010. Searching any of these in the dataset finder brings you back here.

Study design
13 conditions, mostly in triplicate
WT_P8_gubernaculum_biol ×3 KI_P8_gubernaculum_biol ×3 KO_P8_gubernaculum_biol ×3 WT_P23_gubernaculum_biol ×3 KI_P23_gubernaculum_biol ×3 WT_P8_testis_biol ×3 KI_P8_testis_biol ×3 KO_P8_testis_biol ×3 +5 more

Supports a between-group comparison across 39 samples.

How we read this: 13 replicated groups read from 39 sample titles; they account for 39 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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