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Extracellular vesicles from chylomicron-treated endothelial cells drive macrophage inflammation

GSE306274 Mus musculus (mouse) Non-coding RNA profiling 27 samples Submitted 2025/08/25 Platform GPL24247
At a glance

Non-coding RNA profiling · mouse · 27 samples

What it is
Non-coding RNA profilingLevels of RNAs that do not code for proteins, such as miRNA or lncRNA.
Organism
Mus musculus (mouse)
Samples
27 samples: 9 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Extracellular Vesicles From Chylomicron-Treated Endothelial Cells Drive Macrophage InflammationArteriosclerosis, thrombosis, and vascular biology · 2025
Summary (written by the submitters)
Movement of circulating lipids into tissues and arteries requires transfer across the endothelial cell barrier. This process allows the heart to obtain fatty acids (FAs), its chief source of energy, and apolipoprotein B (apoB)-containing lipoproteins to cross the arterial endothelial barrier leading to cholesterol accumulation in the subendothelial space.
Read the full summary ↓
Multiple studies have established elevated postprandial triglyceride-rich lipoproteins (TRLs) as an independent risk factor for cardiovascular disease (CVD). We explored how chylomicrons affect ECs and transfer their FAs across the EC barrier. We had reported that media from chylomicron-treated ECs leads to lipid droplet (LD) formation in macrophages. To determine the responsible component of this media, we assessed whether removing the extracellular vesicles (EVs) would obviate this effect. EVs from control and treated cells were then characterized by protein, lipid and microRNA (miR) content. We also studied the EV-induced transcription changes in macrophages and ECs and whether knockdown of scavenger receptor-BI (SR-BI) altered these responses. In addition, using chylomicrons labeled with [13C]oleate, we studied the uptake and release of this labed by ECs.Chylomicron treatment of ECs led to an inflammatory response that included production of EVs that drove macrophage LD accumulation. The EVs contained little free fatty acids and triglyceride, but abundant phospholipids and diacylglycerols. In concert with this, [13]C labeled chylomicron triglycerides exited ECs primarily in phospholipids. EVs from chylomicron treated versus untreated ECs were larger, more abundant, and contained specific miRs. Treatment of macrophages and naïve ECs with media from chylomicron-treated ECs increased expression of inflammatory genes. EC chylomicron metabolism produces EVs that increase macrophage inflammation and create LDs. Media containing these EVs also increases EC inflammation, illustrating an autocrine inflammatory process. FAs within chylomicron triglycerides are converted to phospholipids within EVs. Thus, EC uptake of chylomicrons constitutes an important pathway for vascular inflammation and tissue lipid acquisition.
Published in
Extracellular Vesicles From Chylomicron-Treated Endothelial Cells Drive Macrophage Inflammation
Tilp A, Nasias D, Carley AL et al. · Arteriosclerosis, thrombosis, and vascular biology 2025 · PMID 41099102 · doi:10.1161/ATVBAHA.125.322712
This dataset
Open GSE306274 in finder → Find more mouse datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE306274_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

Also filed as BioProject PRJNA1310315 and SRA study SRP612564. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41099102) ↗
Study design
9 conditions, mostly in triplicate
Endothelial cell media circulating miRNA, chy… ×3 Endothelial cell media circulating miRNA, con… ×3 Endothelial cell media circulating miRNA, SRB… ×3 Peritoneal macrophages, chylomicron-treated, ×3 Peritoneal macrophages, control, ×3 Peritoneal macrophages, SRBI-knockdown, ×3 Endothelial cells, chylomicron-treated, ×3 Endothelial cells, control, ×3 +1 more

Supports a case/control comparison: 18 samples read as cases, 9 as controls.

How we read this: 9 replicated groups read from 27 sample titles; they account for 27 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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